https://scholars.lib.ntu.edu.tw/handle/123456789/629594
Title: | Development of an assay system for the analysis of host RISC activity in the presence of a potyvirus RNA silencing suppressor, HC-Pro | Authors: | Hong, Syuan-Fei Fang, Ru-Ying Wei, Wei-Lun Jirawitchalert, Supidcha Pan, Zhao-Jun Hung, Yu-Ling Pham, Thanh Ha Chiu, Yen-Hsin Shen, Tang-Long Huang, Chien-Kang SHIH-SHUN LIN |
Keywords: | AGO1 degradation; HC-pro; In vitro RISC assay; T-DNA insertion | Issue Date: | 17-Jan-2023 | Publisher: | BMC | Journal Volume: | 20 | Journal Issue: | 1 | Source: | Virology journal | Abstract: | To investigate the mechanism of RNA silencing suppression, the genetic transformation of viral suppressors of RNA silencing (VSRs) in Arabidopsis integrates ectopic VSR expression at steady state, which overcomes the VSR variations caused by different virus infections or limitations of host range. Moreover, identifying the insertion of the transgenic VSR gene is necessary to establish a model transgenic plant for the functional study of VSR. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/629594 | ISSN: | 1743-422X | DOI: | 10.1186/s12985-022-01956-2 |
Appears in Collections: | 生物科技研究所 |
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