https://scholars.lib.ntu.edu.tw/handle/123456789/630620
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Kuan-Ying A. Huang | en_US |
dc.contributor.author | Zhou, Daming | en_US |
dc.contributor.author | Fry, Elizabeth E. | en_US |
dc.contributor.author | Kotecha, Abhay | en_US |
dc.contributor.author | Huang, Peng Nien | en_US |
dc.contributor.author | Yang, Shu Li | en_US |
dc.contributor.author | Tsao, Kuo Chien | en_US |
dc.contributor.author | Huang, Yhu Chering | en_US |
dc.contributor.author | Lin, Tzou Yien | en_US |
dc.contributor.author | Ren, Jingshan | en_US |
dc.contributor.author | Stuart, David I. | en_US |
dc.date.accessioned | 2023-04-26T01:44:46Z | - |
dc.date.available | 2023-04-26T01:44:46Z | - |
dc.date.issued | 2020-10-16 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/630620 | - |
dc.description.abstract | Enterovirus 71 (EV71)-neutralizing antibodies correlate with protection and have potential as therapeutic agents. We isolate and characterize a panel of plasmablast-derived monoclonal antibodies from an infected child whose antibody response focuses on the plateau epitope near the icosahedral 3-fold axes. Eight of a total of 19 antibodies target this epitope and three of these potently neutralize the virus. Representative neutralizing antibodies 38-1-10A and 38-3-11A both confer effective protection against lethal EV71 challenge in hSCARB2-transgenic mice. The cryo-electron microscopy structures of the EV71 virion in complex with Fab fragments of these potent and protective antibodies reveal the details of a conserved epitope formed by residues in the BC and HI loops of VP2 and the BC and HI loops of VP3 spanning the region around the 3-fold axis. Remarkably, the two antibodies interact with the epitope in quite distinct ways. These plateau-binding antibodies provide templates for promising candidate therapeutics. | en_US |
dc.language.iso | en | en_US |
dc.publisher | NATURE RESEARCH | en_US |
dc.relation.ispartof | Nature Communications | en_US |
dc.subject | NANOLITRE CRYSTALLIZATION EXPERIMENTS; NEUTRALIZING ANTIBODIES; INFECTION; ATTACHMENT; PARTICLES; MECHANISM; CHILDREN; RECEPTOR; DESIGN; SYSTEM | en_US |
dc.title | Structural and functional analysis of protective antibodies targeting the threefold plateau of enterovirus 71 | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1038/s41467-020-19013-3 | - |
dc.identifier.pmid | 33067459 | - |
dc.identifier.scopus | 2-s2.0-85092587640 | - |
dc.identifier.isi | WOS:000582056600016 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85092587640 | - |
dc.relation.pages | 5253 | en_US |
dc.relation.journalvolume | 11 | en_US |
dc.relation.journalissue | 1 | en_US |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.fulltext | with fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | open | - |
crisitem.author.dept | Immunology | - |
crisitem.author.orcid | 0000-0001-6891-6945 | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 免疫學研究所 |
檔案 | 描述 | 大小 | 格式 | |
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s41467-020-19013-3.pdf | Structural and functional analysis of protective antibodies targeting the threefold plateau of enterovirus 71 | 3.57 MB | Adobe PDF | 檢視/開啟 |
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