https://scholars.lib.ntu.edu.tw/handle/123456789/630926
Title: | M2-like macrophages polarized by Foxp3− Treg-of-B cells ameliorate imiquimod-induced psoriasis | Authors: | Huang, Jing Hui Lin, Yu Li LI-CHIEH WANG BOR-LUEN CHIANG |
Keywords: | imiquimod | M2 macrophage | psoriasis | STAT6 | Treg-of-B cells | Issue Date: | 1-Jan-2023 | Publisher: | WILEY | Source: | Journal of Cellular and Molecular Medicine | Abstract: | Our group have demonstrated that splenic B cells contributed to the CD4+CD25− naive T cells conversion into CD4+CD25+Foxp3− regulatory T cells without adding appended cytokines, named Treg-of-B cells which were potent suppressors of adaptive immunity. We like to investigate whether Treg-of-B cells could promote alternatively activated macrophage (M2 macrophages) polarization and alleviate inflammatory disease, psoriasis. In this study, we co-cultured the bone marrow-derived macrophages (BMDMs) with Treg-of-B cells under LPS/IFN-γ stimulation and analyzed the M2-associated gene and protein using qPCR, western blotting, and immunofluorescence staining. We also examined the therapeutic effect of Treg-of-B cell-induced M2 macrophage for skin inflammation using imiquimod (IMQ)-induced psoriatic mouse model. Our results showed that BMDMs co-cultured with Treg-of-B cells upregulated typical M2-associated molecules, including Arg-1, IL-10, Pdcd1lg2, MGL-1, IL-4, YM1/2 and CD206. In an inflammatory environment, TNF-α and IL-6 production by macrophages co-cultured with Treg-of-B cells was decreased significantly. The molecular mechanism revealed that Treg-of-B cells promoted M2 macrophage polarization via STAT6 activation in a cell contact-dependent manner. Moreover, the treatment with Treg-of-B cell-induced M2 macrophages attenuated the clinical manifestations of psoriasis, such as scaling, erythema and thickening in the IMQ-induced psoriatic mouse model. T cell activation in draining lymph nodes was decreased in the Treg-of-B cell-induced M2 macrophage group after IMQ application. In conclusion, our findings suggested that Foxp3− Treg-of-B cells could induce alternatively activated M2 macrophages through STAT6 activation, providing a cell-based therapeutic strategy for psoriasis. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/630926 | ISSN: | 15821838 | DOI: | 10.1111/jcmm.17748 |
Appears in Collections: | 臨床醫學研究所 |
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