https://scholars.lib.ntu.edu.tw/handle/123456789/631029
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Wu, Shin-Da | en_US |
dc.contributor.author | Chuang, Wei-Tsung | en_US |
dc.contributor.author | Ho, Jo-Chen | en_US |
dc.contributor.author | HSUAN-CHEN WU | en_US |
dc.contributor.author | SHAN-HUI HSU | en_US |
dc.date.accessioned | 2023-05-15T03:06:29Z | - |
dc.date.available | 2023-05-15T03:06:29Z | - |
dc.date.issued | 2023-04-12 | - |
dc.identifier.issn | 2073-4360 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/631029 | - |
dc.description.abstract | Self-healing properties, originating from the natural healing process, are highly desirable for the fitness-enhancing functionality of biomimetic materials. Herein, we fabricated the biomimetic recombinant spider silk by genetic engineering, in which Escherichia coli (E. coli) was employed as a heterologous expression host. The self-assembled recombinant spider silk hydrogel was obtained through the dialysis process (purity > 85%). The recombinant spider silk hydrogel with a storage modulus of ~250 Pa demonstrated autonomous self-healing and high strain-sensitive properties (critical strain ~50%) at 25 °C. The in situ small-angle X-ray scattering (in situ SAXS) analyses revealed that the self-healing mechanism was associated with the stick-slip behavior of the β-sheet nanocrystals (each of ~2-4 nm) based on the slope variation (i.e., ~-0.4 at 100%/200% strains, and ~-0.9 at 1% strain) of SAXS curves in the high q-range. The self-healing phenomenon may occur through the rupture and reformation of the reversible hydrogen bonding within the β-sheet nanocrystals. Furthermore, the recombinant spider silk as a dry coating material demonstrated self-healing under humidity as well as cell affinity. The electrical conductivity of the dry silk coating was ~0.4 mS/m. Neural stem cells (NSCs) proliferated on the coated surface and showed a 2.3-fold number expansion after 3 days of culture. The biomimetic self-healing recombinant spider silk gel and thinly coated surface may have good potential in biomedical applications. | en_US |
dc.language.iso | en | en_US |
dc.publisher | MDPI | en_US |
dc.relation.ispartof | Polymers | en_US |
dc.subject | in situ SAXS; recombinant spider silk; self-healing; β-sheet nanocrystal | en_US |
dc.title | Self-Healing of Recombinant Spider Silk Gel and Coating | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.3390/polym15081855 | - |
dc.identifier.pmid | 37112001 | - |
dc.identifier.scopus | 2-s2.0-85154046438 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85154046438 | - |
dc.relation.pages | Article number 1855 | en_US |
dc.relation.journalvolume | 15 | en_US |
dc.relation.journalissue | 8 | en_US |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Biochemical Science and Technology | - |
crisitem.author.dept | Center for Environmental Safety & Health | - |
crisitem.author.dept | Polymer Science and Engineering | - |
crisitem.author.orcid | 0000-0002-7837-1333 | - |
crisitem.author.orcid | 0000-0003-3399-055X | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | Administrative Unit | - |
crisitem.author.parentorg | College of Engineering | - |
顯示於: | 生化科技學系 |
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