https://scholars.lib.ntu.edu.tw/handle/123456789/631181
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Yu, Jiaxin | en_US |
dc.contributor.author | Lin, Yu-Tzu | en_US |
dc.contributor.author | Chen, Wei-Cheng | en_US |
dc.contributor.author | Tseng, Kun-Hao | en_US |
dc.contributor.author | Lin, Hsiu-Hsien | en_US |
dc.contributor.author | Tien, Ni | en_US |
dc.contributor.author | Cho, Chia-Fong | en_US |
dc.contributor.author | Huang, Jhao-Yu | en_US |
dc.contributor.author | Liang, Shinn-Jye | en_US |
dc.contributor.author | Ho, Lu-Ching | en_US |
dc.contributor.author | Hsieh, Yow-Wen | en_US |
dc.contributor.author | Hsu, Kai-Cheng | en_US |
dc.contributor.author | Ho, Mao-Wang | en_US |
dc.contributor.author | PO-REN HSUEH | en_US |
dc.contributor.author | Cho, Der-Yang | en_US |
dc.date.accessioned | 2023-05-19T06:13:31Z | - |
dc.date.available | 2023-05-19T06:13:31Z | - |
dc.date.issued | 2023-03-31 | - |
dc.identifier.issn | 09248579 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/631181 | - |
dc.description.abstract | The objective of this study was to develop a rapid prediction method for carbapenem-resistant Klebsiella pneumoniae (CRKP) and colistin-resistant K. pneumoniae (ColRKP) based on routine MALDI-TOF mass spectrometry (MS) results in order to formulate a suitable and rapid treatment strategy. A total of 830 CRKP and 1462 carbapenem-susceptible K. pneumoniae (CSKP) isolates were collected; 54 ColRKP isolates and 1592 colistin-intermediate K. pneumoniae (ColIKP) isolates were also included. Routine MALDI-TOF MS, antimicrobial susceptibility testing, NG-Test CARBA 5, and resistance gene detection were followed by machine learning (ML). Using the ML model, the accuracy and area under the curve for differentiating CRKP and CSKP were 0.8869 and 0.9551, respectively, and those for ColRKP and ColIKP were 0.8361 and 0.8447, respectively. The most important MS features of CRKP and ColRKP were m/z 4520-4529 and m/z 4170-4179, respectively. Of the CRKP isolates, MS m/z 4520-4529 was a potential biomarker for distinguishing KPC from OXA, NDM, IMP, and VIM. Of the 34 patients who received preliminary CRKP ML prediction results (by texting), 24 (70.6%) were confirmed to have CRKP infection. The mortality rate was lower in patients who received antibiotic regimen adjustment based on the preliminary ML prediction (4/14, 28.6%). In conclusion, the proposed model can provide rapid results for differentiating CRKP and CSKP, as well as ColRKP and ColIKP. The combination of ML-based CRKP with preliminary reporting of results can help physicians alter the regimen approximately 24 h earlier, resulting in improved survival of patients with timely antibiotic intervention. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | International journal of antimicrobial agents | en_US |
dc.subject | Carbapenem-resistant Klebsiella pneumoniae; Carbapenemase-producing Klebsiella pneumoniae; Colistin resistance; MALDI-TOF MS; Machine learning; Prediction platform | en_US |
dc.title | Direct prediction of carbapenem-resistant, carbapenemase-producing, and colistin-resistant Klebsiella pneumoniae isolates from routine MALDI-TOF mass spectra using machine learning and outcome evaluation | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.ijantimicag.2023.106799 | - |
dc.identifier.pmid | 37004755 | - |
dc.identifier.scopus | 2-s2.0-85153337996 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85153337996 | - |
dc.relation.journalvolume | 61 | en_US |
dc.relation.journalissue | 6 | en_US |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Laboratory Medicine | - |
crisitem.author.dept | Laboratory Medicine-NTUH | - |
crisitem.author.orcid | 0000-0002-7502-9225 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學院附設醫院 (臺大醫院) |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。