https://scholars.lib.ntu.edu.tw/handle/123456789/632977
Title: | Dual-Function Fibrous Co-Polypeptide Scaffolds for Neural Tissue Engineering | Authors: | Ma, Tienli Yang, Shangchih SHYH-CHYANG LUO WEI-LI CHEN SHU-LANG LIAO Su, Weifang |
Keywords: | aligned fiber | co-polypeptide | glutamate | lysine | neurite | tissue engineering | Issue Date: | 1-Feb-2023 | Publisher: | WILEY-V C H VERLAG GMBH | Journal Volume: | 23 | Journal Issue: | 2 | Source: | Macromolecular Bioscience | Abstract: | This paper reports dual-function (high cell attachment and cell viability) fibrous scaffolds featuring aligned fibers, displaying good biocompatibility and no cytotoxicity. These scaffolds are fabricated through the electrospinning of a co-polypeptide comprising molar equivalents of N6-carbobenzyloxy-l-lysine and γ-benzyl-l-glutamate, with the lysine moieties enhancing cell adhesion and the neural-stimulating glutamate moieties improving cell viability. These new scaffolds allow neural cells to attach and grow effectively without any special surface treatment or coating. Pheochromocytoma (PC-12) cells grown on these scaffolds exhibit better neuronal activity and longer neurite length, relative to those grown on scaffolds prepared from their respective homo-polypeptides. When the scaffolds are partially hydrolyzed such that they present net positive charge and increased hydrophilicity, the cell viability and neurite growth both increase further. Accordingly, these novel co-polypeptide fibrous scaffolds have potential applications in neural tissue engineering. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/632977 | ISSN: | 16165187 | DOI: | 10.1002/mabi.202200286 |
Appears in Collections: | 材料科學與工程學系 |
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