https://scholars.lib.ntu.edu.tw/handle/123456789/633145
標題: | ARMS-NF-κB signaling regulates intracellular ROS to induce autophagy-associated cell death upon oxidative stress | 作者: | YI-HUA LIAO Wu, June-Tai Hsieh, I-Chun HSIU-HSIANG LEE PEI-HSIN HUANG |
關鍵字: | Biological sciences;Cell biology;Molecular biology | 公開日期: | 17-二月-2023 | 出版社: | Elsevier Inc. | 卷: | 26 | 期: | 2 | 來源出版物: | iScience | 摘要: | Ankyrin repeat-rich membrane spanning (ARMS) plays roles in neural development, neuropathies, and tumor formation. Such pleiotropic function of ARMS is often attributed to diverse ARMS-interacting molecules in different cell context. However, it might be achieved by ARMS' effect on global biological mediator like reactive oxygen species (ROS). We established ARMS-knockdown in melanoma cells (siARMS) and in Drosophila eyes (GMR>dARMS RNAi ) and challenged them with H2O2. Decreased ARMS in both systems compromises nuclear translocation of NF-κB and induces ROS, which in turn augments autophagy flux and confers susceptibility to H2O2-triggered autophagic cell death. Resuming NF-κB activity or reducing ROS by antioxidants in siARMS cells and GMR>dARMS RNAi fly decreases intracellular peroxides level concurrent with reduced autophagy and attenuated cell death. Conversely, blocking NF-κB activity in wild-type flies/melanoma enhances ROS and induces autophagy with cell death. We thus uncover intracellular ROS modulated by ARMS-NFκB signaling primes autophagy for autophagic cell death upon oxidative stress. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/633145 | ISSN: | 25890042 | DOI: | 10.1016/j.isci.2023.106005 |
顯示於: | 醫學系 |
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