https://scholars.lib.ntu.edu.tw/handle/123456789/635395
Title: | Lilium Gray Mold Suppression Conferred by the Host Antimicrobial Protein LsGRP1 Involves Main Pathogen-Targeted Manipulation of the Nonantimicrobial Region LsGRP1N | Authors: | Lin, Chia-Hua Liu, Fang-Wei Pan, Ying-Chieh CHAO-YING CHEN |
Keywords: | Botrytis elliptica; antimicrobial sensitivity; host defense elicitation; hyphal growth; infection-related gene expression; pathogen manipulation; spore germination | Issue Date: | 30-Aug-2023 | Journal Volume: | 71 | Journal Issue: | 34 | Source: | Journal of agricultural and food chemistry | Abstract: | Antimicrobial protein LsGRP1 protects Lilium from gray mold mainly caused by the destructive pathogen Botrytis elliptica; however, its nonantimicrobial region LsGRP1N conversely promotes spore germination of this fungus. By assaying the effects of LsGRP1N, LsGRP1, and the combination of LsGRP1N and the antimicrobial region LsGRP1C on fungal spore germination, hyphal growth, and Lilium gray mold development, LsGRP1N was found to improve the LsGRP1C sensitivity of B. elliptica and disease suppression by LsGRP1C. B. elliptica cell vitality assays indicated that LsGRP1N pretreatment uniquely enhanced the lethal efficiency of LsGRP1C compared to the control peptides. In addition, LsGRP1N-treated B. elliptica was demonstrated to lower infection-related gene expression and increase host-defense-eliciting activity, as indicated by reverse transcription quantitative polymerase chain reaction and histochemical-staining-based callose detection results, respectively. Therefore, LsGRP1N showed a novel mode of action for antimicrobial proteins by manipulating the main pathogen, which facilitated the development of target-specific and dormant microbe-eradicating antimicrobial agents. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/635395 | ISSN: | 00218561 | DOI: | 10.1021/acs.jafc.3c04221 |
Appears in Collections: | 植物病理與微生物學系 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.