https://scholars.lib.ntu.edu.tw/handle/123456789/637482
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lee, Chih-Ying | en_US |
dc.contributor.author | WEN-FANG CHENG | en_US |
dc.contributor.author | PO-HAN LIN | en_US |
dc.contributor.author | YU-LI CHEN | en_US |
dc.contributor.author | Huang, Shih-Han | en_US |
dc.contributor.author | Lei, Kai-Hang | en_US |
dc.contributor.author | Chang, Ko-Yu | en_US |
dc.contributor.author | Ko, Min-Yu | en_US |
dc.contributor.author | HUNG-YUAN CHI | en_US |
dc.date.accessioned | 2023-12-04T01:48:22Z | - |
dc.date.available | 2023-12-04T01:48:22Z | - |
dc.date.issued | 2023-11-21 | - |
dc.identifier.issn | 26663791 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/637482 | - |
dc.description.abstract | Homologous recombination (HR)-mediated DNA repair is a prerequisite for maintaining genome stability. Cancer cells displaying HR deficiency (HRD) are selectively eliminated by poly(ADP-ribose) polymerase inhibitors (PARPis). To date, sequencing of HR-associated genes and analyzing genome instability have been used as clinical predictions for PARPi therapy. However, these genetic tests cannot reflect dynamic changes in the HR status. Here, we have developed a virus- and activity-based functional assay to quantify real-time HR activity directly. Instead of focusing on a few HR-associated genes, our functional assay detects endpoint HR activity and establishes an activity threshold for identifying HRD across cancer types, validated by PARPi sensitivity and BRCA status. Notably, this fluorescence-based assay can be applied to primary ovarian cancer cells from patients to reflect their level of HRD, which is associated with survival benefits. Thus, our work provides a functional test to predict the response of primary cancer cells to PARPis. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Cell Press | en_US |
dc.relation.ispartof | Cell reports. Medicine | en_US |
dc.subject | BRCA; DNA repair activity; DNA repair deficiency; HRD; PARP inhibitor; breast cancer; colorectal cancer; functional test; ovarian cancer | en_US |
dc.title | An activity-based functional test for identifying homologous recombination deficiencies across cancer types in real time | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1016/j.xcrm.2023.101247 | - |
dc.identifier.pmid | 37863059 | - |
dc.identifier.scopus | 2-s2.0-85176445638 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/85176445638 | - |
dc.relation.pages | Article number 101247 | en_US |
dc.relation.journalvolume | 4 | en_US |
dc.relation.journalissue | 11 | en_US |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Oncology | - |
crisitem.author.dept | Surgery-NTUCC | - |
crisitem.author.dept | Medical Genetics-NTUH | - |
crisitem.author.dept | Medical Genomics and Proteomics | - |
crisitem.author.dept | Obstetrics & Gynecology | - |
crisitem.author.dept | Obstetrics & Gynecology-NTUH | - |
crisitem.author.dept | Obstetrics&Gynecology-NTUHYL | - |
crisitem.author.dept | Biochemical Sciences | - |
crisitem.author.dept | College of Life Science | - |
crisitem.author.orcid | 0000-0002-3282-6304 | - |
crisitem.author.orcid | 0000-0001-6066-398X | - |
crisitem.author.orcid | 0000-0002-9742-4752 | - |
crisitem.author.orcid | 0000-0001-9229-8729 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Cancer Center (NTUCC) | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital Yun-Lin Branch | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | National Taiwan University | - |
顯示於: | 生化科學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。