DC 欄位 | 值 | 語言 |
dc.contributor | 彭慶安 | zh-TW |
dc.contributor | 臺灣大學:化學工程學研究所 | zh-TW |
dc.contributor.author | 徐逸修 | zh-TW |
dc.contributor.author | Hsu, Yi-Shiou | en |
dc.creator | 徐逸修 | zh-TW |
dc.creator | Hsu, Yi-Shiou | en |
dc.date | 2008 | en |
dc.date.accessioned | 2010-06-30T06:29:12Z | - |
dc.date.accessioned | 2018-06-28T17:17:10Z | - |
dc.date.available | 2010-06-30T06:29:12Z | - |
dc.date.available | 2018-06-28T17:17:10Z | - |
dc.date.issued | 2008 | - |
dc.identifier.other | U0001-1907200821014100 | en |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/187006 | - |
dc.description.abstract | 在本研究中,首先我們合成硒化鎘/硫化鋅 (CdSe/ZnS) 量子點,並利用調整長晶時間來調控合成之量子點粒徑及螢光,藉此得到我們所需的量子點。但為了使量子點能應用於生物系統上必須將量子點再作進一步的表面修飾,才能使量子點能溶在水中並保有其螢光特性,相較於早期的配位基置換法 (ligand exchange) 易造成表面官能基置換不完全,我們採用的方法是利用生物相容性較高的多醣體褐藻酸經官能基修飾成兩相性褐藻酸去包覆有機相量子點。先以過碘酸鈉的IO-4來氧化褐藻酸上的相鄰氫氧基 (1,2-diol) 而形成醛基 (aldehyde group) ; 再於醛基官能基上接上辛胺 (octylamine) 形成一個二相性褐藻酸。在辛胺上的長鏈烷基 (alkyl chain) 會藉由疏水作用力而插入量子點外圍三辛基氧化磷(TOPO)之疏水基團空隙;而在褐藻酸另一端上的羧基 (COOH) 則能使包覆量子點後的水溶性量子點穩定地存在於水中。著再利用帶正電高分子將水溶性量子點與登革熱病毒藉由靜電吸引力而形成量子點-病毒複合體。因量子點之螢光特性,使病毒能夠在共軛焦顯微鏡下藉由量子點的螢光來觀察。利用此一新型的量子點/病毒複合體可以快速分析病毒感染細胞的情況。為驗証此複合體之功能,由螺旋藻中萃取出之異藻藍蛋白 (allophycocyanin, APC) 先加入BHK-21細胞中一小時。之後將培養液更換為含有量子點/病毒複合體之培養液,並於不同的時間點下觀察細胞。結果顯示共軛焦顯微鏡下的螢光會隨著APC濃度之增加而明顯下降; 亦即APC對於抑制登革熱病毒進入BHK-21細胞具有明顯的效果。相比於現今做病毒感染細胞之檢測大多需3-7天,本研究發展出來的細胞影像分析方法,可作為快速篩選抗登革熱病毒藥物的平台。 | zh-TW |
dc.description.abstract | In this study, CdSe/ZnS quantum dots (QDs) with various sizes and luminescent colors were synthesized in organic phase by modulating the growth time of semiconductor nanocrystals. For the purpose of applying QDs in biological environments, surface modification of QD rendering its water soluble and fluorescence intact is of utmost importance. In comparison with ligand exchange method which causes incomplete surface coverage, highly biocompatible polysaccharide sodium alginate was harnessed to synthesize amphiphilic alginate surfactant that is used to encapsulate QDs. Oxidation reaction on the diol group at C-2 and C-3 positions of the uronic units of sodium alginate was performed with sodium periodate to form aldehyde groups; then the alkyl moieties were introduced by reacting the aldehyde group of 2,3-dialdehydic alginate with octylamine via reductive amination. The alkyl chains of amphiphilic alginate (AA) are intercalate with the hydrophobic surfactant (tri-octyl-phosphine oxide) on the surface of QDs through hydrophobic attraction; while the carboxylic groups of amphiphilic alginate can make the encapsulated QDs (AA-QDs) stably dispersed in the aqueous milieu.e further employed polycationic polybrene to neutralize the electrostatic repulsion force between both negatively charged AA-QDs and dengue virus and form QD/dengue virus complexes. By virtue of the fluorescence of QDs, dengue virus can be monitored by fluorescent microscopy. This innovative QD/dengue virus complexes can be used to fast analyze the status of viral infection to susceptible cells. In order to demonstrate the capability of QD/dengue virus complexes, allpophycocyanin (APC) extracted from Spirulina sp. incubated with BHK-21 cells for 1 hour and then replaced with culture medium containing the QD/dengue virus complexes for various time intervals. Our results showed that the fluorescence of QD/dengue virus complexes under confocal microscopy decreased along with the increase of APC concentration. In other words, APC is a potential anti-viral agent for preventing BHK-21 cells from dengue virus infection. Compared with current methods used for diagnosizingvirus infection taking 3~7 days, the cell-image based screening analysis anti-dengue viral developed here can be a platform for fast screening of anti-dengue viral agents. | en |
dc.description.tableofcontents | Index of Figures iiindex of Tables ixndex of Flow Charts xbstract xibstract (Chinese) xiiihapter 1 General Review 1.1. Quantum size effects and semiconducting nanocrystals 3.2. Nanotechnology in bio-related field 6.3. Quantum Dot as luminescent nanocrystals 8.4. Biological applications of luminescene quantum dot 10.5. Amphiphilic Approach to quantum dot 14.6. Amphiphilic alginate surfactant 16.7. Virus-tagged with quantum dot 19.8. Dengue virus 22hpater 2 Materials and Instrument 25.1. Materials 25.2. Instrument 28hpater 3 Experimental 31.1. Synthesis of CdSe/ZnS quantum dot. 32.2. Preparation of amphiphilic alginate surfactant 38.3. Quantum dot encapsulated with amphiphilic alginate 41.4. BHK-21 cell and dengue virus 43.5. Inhibition of QD/virus infection by microalgae extracts 43.6. Measurement 45.6.1. Particle size analysis 45.6.2. UV/VIS and photoluminescence spectrophotometry 45.6.3. Transmission electron microscopy 46.6.4. Zeta potential 46.1 Quantum Dot encapsulated with amphiphilic alginate 47.2. Cell-QD Interaction 52.3 Effect of polybrene on infectivity of QD-virus 55.4 Antiviral activity of allophycocyanin 59hapter 5 Conclusion 62hapter 6 Suggested Furture Work 63hapter 7 References 65ppendices 77ppdedix 1-Poster presentation at the 54th annual conference of TwIChE, chang gung university, Tao-Yuan, Taiwan. (2007) 77ppdedix 2-Poster presentation at the 10th world congress on biosensors, Shanghai International Convention Centre, Shanghai, China. (2008) 78ppdedix 3-Oral at the 13th annual conference of biochemical engineering,yuan ze university, Chung-Li, Taiwan. (2008) 79 | en |
dc.format.extent | 136 bytes | - |
dc.format.mimetype | text/html | - |
dc.language | en | en |
dc.language.iso | en_US | - |
dc.subject | 褐藻酸 | zh-TW |
dc.subject | 量子點 | zh-TW |
dc.subject | 病毒 | zh-TW |
dc.subject | Quantum dots | en |
dc.subject | dengue virus | en |
dc.subject | amphiphilic alginate | en |
dc.subject.classification | [SDGs]SDG3 | - |
dc.title | 製備兩相性褐藻酸包覆之水溶性量子點用於登革熱病毒藥物之篩選 | zh-TW |
dc.title | Preparation of Quantum Dots Encapsulated with Amphiphilic lginate for Screening Anti-Dengue Viral Agents | en |
dc.type | thesis | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/187006/1/index.html | - |
item.openairetype | thesis | - |
item.fulltext | with fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_46ec | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en_US | - |
item.cerifentitytype | Publications | - |
顯示於: | 化學工程學系
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