https://scholars.lib.ntu.edu.tw/handle/123456789/641597
Title: | Secretome from Magnetically Stimulated Muscle Exhibits Anticancer Potency: Novel Preconditioning Methodology Highlighting HTRA1 Action | Authors: | Tai, Yee Kit Iversen, Jan Nikolas Chan, Karen Ka Wing Fong, Charlene Hui Hua Abdul Razar, Rafhanah Banu Ramanan, Sharanya Yap, Lye Yee Jasmine Yin, Jocelyn Naixin Toh, Shi Jie Wong, Craig Jun Kit Koh, Pei Fern Angele RUBY YUN-JU HUANG Franco-Obregón, Alfredo |
Keywords: | PEMFs; aminoglycoside antibiotics; breast cancer; conditioned media; exercise; exerkines; metastasis; myokines; proteomics; soluble biomarkers | Issue Date: | 5-Mar-2024 | Journal Volume: | 13 | Journal Issue: | 5 | Source: | Cells | Abstract: | Briefly (10 min) exposing C2C12 myotubes to low amplitude (1.5 mT) pulsed electromagnetic fields (PEMFs) generated a conditioned media (pCM) that was capable of mitigating breast cancer cell growth, migration, and invasiveness in vitro, whereas the conditioned media harvested from unexposed myotubes, representing constitutively released secretome (cCM), was less effective. Administering pCM to breast cancer microtumors engrafted onto the chorioallantoic membrane of chicken eggs reduced tumor volume and vascularity. Blood serum collected from PEMF-exposed or exercised mice allayed breast cancer cell growth, migration, and invasiveness. A secretome preconditioning methodology is presented that accentuates the graded anticancer potencies of both the cCM and pCM harvested from myotubes, demonstrating an adaptive response to pCM administered during early myogenesis that emulated secretome-based exercise adaptations observed in vivo. HTRA1 was shown to be upregulated in pCM and was demonstrated to be necessary and sufficient for the anticancer potency of the pCM; recombinant HTRA1 added to basal media recapitulated the anticancer effects of pCM and antibody-based absorption of HTRA1 from pCM precluded its anticancer effects. Brief and non-invasive PEMF stimulation may represent a method to commandeer the secretome response of muscle, both in vitro and in vivo, for clinical exploitation in breast and other cancers. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/641597 | ISSN: | 2073-4409 | DOI: | 10.3390/cells13050460 |
Appears in Collections: | 醫學系 |
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