綠色材料--生物可分解聚脂材料PHA生產、應用與分解技術開發─子計畫一：由微生物發酵生產功能性PHA及其形態探討(II)
Date Issued
2003
Date
2003
Author(s)
黃世佑
DOI
912621Z002013
Abstract
The polyhydroxyalkanoates (PHA) polymers has many advantages. In this work,
a shake flask method was used to produce PHAs by cultivating a halobacterium
Haloferax mediterranei. The structure of PHAs was determined by NMR and FTIR.
The substrates used were glucose, glutaric acid, 4-pentenoic acid and
5-phenylvaleric acid. Sampling in a time interval, to analyze the structure of products
for structual analysis and determination of properties. The conditions to obtain the
PHA possessing functional groups were investigated. Tm of PHBV copolymer
exhibited a shoulder.On the other hand, pure PHB did not exhibit the shoulder.
The Tm and Tg of commercial PHAs deceases with the increase of HV
(hydroxyvalerate) proportion.
The PHAs obtained with glucose as a sole carbon source were primarily PHBV8.
The ratio of HB and HV in the product of fermentation supplemented with
4-pentenoic acid was almost the same.Its structure was PHBV8.
The ratio of HV as 5-phenylvaleric acid was fed into a fermentation system
increased with the fermentation time. It increased up to 16%. There was a high HV
content (18%) in fermentation broth when glutaric acid was fed into the fermentation
system.
Subjects
Haloferax mediterranei
PHA
PHBV
functional group
Glutaric acid
4-Pentenoic acid
5-Phenylvaleric acid
Publisher
臺北市：國立臺灣大學化學工程學系暨研究所
Type
report
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