https://scholars.lib.ntu.edu.tw/handle/123456789/79316
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor | 張正憲 | en |
dc.contributor | 臺灣大學:應用力學研究所 | zh_TW |
dc.contributor.author | 黃俊凱 | zh |
dc.contributor.author | Huang, Chun-Kai | en |
dc.creator | 黃俊凱 | zh |
dc.creator | Huang, Chun-Kai | en |
dc.date | 2004 | en |
dc.date.accessioned | 2007-11-29T02:17:45Z | - |
dc.date.accessioned | 2018-06-29T00:06:15Z | - |
dc.date.available | 2007-11-29T02:17:45Z | - |
dc.date.available | 2018-06-29T00:06:15Z | - |
dc.date.issued | 2004 | - |
dc.identifier | zh-TW | en |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/62486 | - |
dc.description.abstract | 本文利用原子力顯微鏡來量測黏質色拉雷菌(S.M.菌)之野生菌CH1與突變株O3,在形成生物膜後其表面之楊氏係數、軟硬度與黏附力的大小,並討論細菌生物膜形成的時間與其楊氏係數、軟硬度、和黏附力之關係。在本文中將生物膜形成的時間控制為1小時、3小時和5小時來量測菌體表面的各種力學性質,其結果顯示CH1與O3形成生物膜後,隨時間增加菌體表面的楊氏模數、硬度與黏附力也會有增加的趨勢。而在黏附力的量測中發現O3菌體表面的黏附力小於CH1菌體表面,因為O3缺少O抗原以致形成生物膜後所分泌的多醣類較少而產生較小的黏附力。 | zh_TW |
dc.description.tableofcontents | 摘要 Ⅰ 目錄 Ⅱ 圖目錄 Ⅳ 表目錄 ⅩⅣ 符號說明 ⅩⅤ 第一章 前言 1 1-1序論 1 1-2 文獻回顧 3 1-3研究動機 6 第二章 實驗系統與方法 8 2-1原子力顯微鏡基本架構 8 2-2原子力顯微鏡的量測模式 11 2-2-1 力-距離曲線 14 2-2-2 比例常數量測 15 2-2-3 懸臂樑彈性常數(spring constant)校正 17 2-2-4樣本形變量 的量測 18 2-3 生物樣本特性與製備 19 2-3-1 生物膜介紹 19 2-3-2野生S.M.菌CH1與突變株O3介紹 20 2-3-3 樣本配置 22 2-4 理論模型 23 2-4-1楊氏模數分析 23 2-4-2 軟硬度分析 25 2-4-3 黏附力分析 26 第三章 實驗結果與討論 28 3-1 菌體外觀與尺寸分析 28 3-2 楊氏模數分析 32 3-3 硬度分析 36 3-4 黏附力分析 39 第四章 結論與未來展望 44 4-1 結論 44 4-2 未來展望 45 參考文獻 46 附錄 49 附圖(Experimental raw data) 60 | zh_TW |
dc.language | zh-TW | en |
dc.language.iso | en_US | - |
dc.subject | 原子力顯微鏡 | en |
dc.subject | S.M.菌 | en |
dc.subject | 力學性質 | en |
dc.subject | Atomic force microscopy | en |
dc.subject | mechanical properties | en |
dc.subject | S.M. bacteria | en |
dc.title | 用原子力顯微術探討DNA修飾對S.Marcescens菌落力學性質之影響 | zh |
dc.title | Detect the mechanical properties of Differential DNA in S.M. Bacteria Colony with Atomic Force Microscopy | en |
dc.type | thesis | en |
dc.relation.reference | [1] G. Binnig, C. F. Quate, and C. Gerber, Phys. Rev. Lett 1986. 56:930 [2] 陳吉良、林世明、林良平, 原子力顯微術及其在微生物學上之應用.科儀新知 139第25卷第五期 p74-81 [3] Xu W, Mulhern PJ, Blackford BL, Jericho MH, Firtel M, Beveridge TJ:Modeling and Measuring the Elastic Properties of an Archaeal Surface , the Sheath of Methanospirillum hungatei,and the Implication for Methane Production. Journal of Bacteriology 1996.178:3106-3112 [4] X.Yao, M. JERICHO, D. PINK, and T. BEVERIDGE:Thickness and Elasticity of Gram-Negative Murin Sacculi Measured by Atomic Force Microscopy. Journal of bacteriology 1999, 181:6865-6875 [5] Ahmed Touhami, Bernard Nysten, and Yves F.Dufrene: Nanoscale Mapping of the Elasticity of Microbial Cells by Atomic Force Microscopy. Langmuir 2003, 19:4539-4543. [6] Herbert H.P. Fang, Kwong-Yu Chan, Li-Chong Xu:Quantification of Bacterial Adhesion Force Using Atomic Force Microscopy. Journal of Microbiological Methods 2000. 40:89-97 [7] Prisca Schaer-Zammaretti, Job Ubbink:Imaging of lactic acid bacteria with AFM-elasticity and adhesion maps and their relationship to biological and structural data. Ultramicroscopy 2003. 97:199-208. [8] Nehal I. Abu-Lail and Terri A. Camesano: Role of Lipopolysaccharides in the Adhesion, Retention, and Transport of Escherichia coli JM109. Environ. Sci. Technol. 2003. 37:2173-2183 [9] Morris, V. J., Kirby, A. R., Gunning, A. P., Atomic Force Microscopy for Biologists, Imperial College Press: London, 1999. [10] 黃惠忠等, 2003,“奈米材料分析”化學工業出版社 [11] J. P Cleveland, S. Manne, D. Bocek, P. K. Hansma:A nondestructive method for determining the spring constant of cantilevers for scanning force microscopy. Rev. Sci. Instrum. 1993,64(2):403-405 [12] Albrecht L Weisenhornt, Mitra Khorsandit, Sandor Kasaat, Vassilis Gotzost and Hans-Jurgen Butt: Deformation and height anomaly of soft surfaces studied with an AFM. Nanotechnology 1993,4:106-113 [13] Yea-Ling Ong, Anneta Razatos, George Georgiou, and Mukul M. Sharma:Adhesion force between E. coli bacteria and biomaterial surface. Langmuir 1999. 15:2719-2725 [14] 蔡文城, 2002, “微生物學”藝軒圖書 [15] J.W. Harding and I.N. Sneddon: The Elastic Stresses Produced by the Plane Surface of a Semi-Infinite Elastic Solid by a Rigid Punch.Proc. Cambridge Phil. Soc.1946.41:16-26 [16] A. Vinckier and G. Semenza: Measuring Elasticity of Biological Materials by Atomic Force Microscopy. FEBS Letters 1998.430:12-16 [17] http://www.bact.wisc.edu/MicrotextBook/BacterialStructure/CellWall.html | zh_TW |
item.openairecristype | http://purl.org/coar/resource_type/c_46ec | - |
item.openairetype | thesis | - |
item.languageiso639-1 | en_US | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
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