Detection of Photobacterium damselae subsp. Piscicida by a Piezoresistive Microcantilever Biosensor
Date Issued
2009
Date
2009
Author(s)
Hung, Wei-Ting
Abstract
Aquaculture industry has gained prosperous and rapid development in Taiwan recently, in which the monitoring of water quality is an essential part of fish protection. When fish are being bred in seawater with bacteria concentration that is higher than standard value, mortality rises. Therefore, detection of bacteria in seawater with a quantitative, qualitative, and real-time monitoring method becomes an important issue. Cobia is one of high economical value aquaculture fish in Taiwan and is being seriously threaten by Photobacterium damselae subsp. Piscicida(Pdp.). If the infected fish is not being treated in time, it might lead to Pseudotuberculosis and will soon be decreased.resently, most biosensors are using chemical or fluorescence labeling for bacteria detection. For the purpose of label-free sensing, this study combines the mechanical theory and the transduction of bio-signal, and utilizes the surface stress change induced by the recognition of bio-molecule for on-site real time detection. The signal is presented as voltage output and is analyzed in the computer after amplification. This work shows the real reaction of quantitative and qualitative results capable of distinguishing various concentrations of Pdp. in liquid by the piezoresistive microcantilever. The sensing limitation is about 2?104 cfu/ml. Also, the SEM is used to observe the binding state of Pdp. onto the sensing surface of the microcantilever. In comparison to the resonant frequency microcantilever biosensors, the piezoresistive microcantilever biosensors have advantages over rapid detection and on-site real-time detection.
Subjects
Microcantilever
Piezoresistive
Surface stress
Photobacterium damselae subsp. Piscicida
Microelectromechanical system(MEMS)
SDGs
Type
thesis
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