DC 欄位 | 值 | 語言 |
dc.contributor | 李雨 | zh-TW |
dc.contributor | 臺灣大學:應用力學研究所 | zh-TW |
dc.contributor.author | 馮德威 | zh-TW |
dc.contributor.author | Fung, Tak-Wai | en |
dc.creator | 馮德威 | zh-TW |
dc.creator | Fung, Tak-Wai | en |
dc.date | 2009 | en |
dc.date.accessioned | 2010-05-18T04:58:43Z | - |
dc.date.accessioned | 2018-06-29T00:16:10Z | - |
dc.date.available | 2010-05-18T04:58:43Z | - |
dc.date.available | 2018-06-29T00:16:10Z | - |
dc.date.issued | 2009 | - |
dc.identifier.other | U0001-2907200918180200 | en |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/183555 | - |
dc.description.abstract | 本論文利用微機電技術製造旅波式介電泳幫浦及細胞分離晶片。旅波式介電泳幫浦為一截面為矩形的流道,在其中一壁面鋪上如鐵軌般垂直於流道的電極,並施以六組四相位差的交流訊號,此時紅血球將會被傳統式介電泳力抬離電極表面,旅波式介電泳力則會驅動紅血球往相位遞增的方向前進,血漿則會藉由紅血球移動時的黏滯阻力被帶動,而達到傳輸血液的效果;細胞分離晶片亦使用旅波式介電泳力,當細胞於同一位置出發,經過與流道具有特定傾斜角度的電極陣列,會產生不同幅度的偏向,即可分辨出不同的細胞。本文針對這兩部份的研究,完成的工作如下:(1)找出最佳的旅波式介電泳幫浦操作頻率,5MHz時全血平均流速可達50μm/s。(2)比較在首根電極前增加兩根輔助電極於不同情況下是否能提升幫浦的效能,結果主要顯示輔助電極可助縮短幫浦達穩態運輸時的暫態時間,如10MHz時由30分鐘縮短為20分鐘。(3)探討了血液濃度對旅波式介電泳幫浦的影響。(4)測試幾種不同的流道設計,電極區漸縮的流道有較好的表現,幫浦平均流速從原本的11.1μm/s提升至16.5μm/s。(5)本研究設計的細胞分離晶片,可在10MHz、背景流速69.44~148.81μm/s時,將RPMI培養液中的CL1-0、CL1-5和白血球分辨出來;在10MHz、背景流速49.60~128.97μm/s時,將PBS緩衝液中的Jurkat、白血球和紅血球分辨出來。 | zh-TW |
dc.description.abstract | MEMS techniques are emploged to fabricate travelling wave dielectrophoretic (TWDEP) pumps and cell separation chips. The pump is a micro channel with a rectangular cross-section with an electrode array on one of its walls. The electrodes are operated under ac signal 90º shift of between neighboring electrodes, and there are 24 basic electrodes on an array. The negative dielectrophoretic force repels the red blood cells from the electrode surface and the travelling wave dielectrophoretic force drives the cells along the direction of increasing phase. The plasma will be drag through viscous force as the red blood cells move, and the whole blood is delivered. In cell separation chips, the paths of different cells in a background flow are deflected by a designed travelling wave dielectrophoresis, and thus different cells are separated. Several works have been completed: (1) Optimum frequency for whole blood delivery in travelling wave dielectrophoretic pumps is found to be around 5MHz. (2) Two assistant electrodes before the basic electrode array can shorten the transient time of the TWDEP pump, and can enhance pump performance in some cases. (3) Performance of the TWDEP pump at different volume ratios of cells have been studied. (4) Different channel designs were carried out, and it is found that electrode array in converging channel section has better pump performance, the average velocity increases from 11.1μm/s promote to the 16.5μm/s. (5) At 10MHz and background velocity 69.44~148.81μm/s , the cell separation chip can separate CL1-0, CL1-5 and WBC in the RPMI medium ; At and background velocity 49.60~128.97μm/s, our cell separate chip can recognize Jurkat, WBC and RBC in the PBS solution. | en |
dc.description.tableofcontents | 誌謝 i文摘要 ii文摘要 iii錄 iv表目錄 vii一章 緒論 1.1 研究動機及背景 1.2 研究目的 2.3 文獻回顧 2.4 本文架構 4二章 理論 5 2-1 有效偶極矩 5-2 介電泳力 6-3 等效圓球模型 7-4 正負介電泳力發生之原因 8-5 CM factor與全血傳輸性能的關係 9-6 黏滯拖曳力 10三章 實驗方法與設備 12.1 系統設計 12.2 運用MEMS技術製造電極晶片 12-2-1 清潔 12-2-2 電極蒸鍍 13-2-3 電極微影製程 13.3 運用MEMS技術製造微流道 14-3-1 流道微影製程 14-3-2 PDMS翻模製作 15.4 元件接合與外部連結方式 16.5 實驗設備 16.6 速度量測 17.7 生物粒子的選取 18-7-1 貼附型細胞的收集與次培養 19-7-2 純化白血球濃厚液流程 19.8 溶液的選取 19-8-1 貼附型細胞培養液的調配與保存 19.9 細胞正負介電泳力測試 20四章 結果與討論 21 4.1 旅波式介電泳幫浦 21-1-1 不同頻率與樣本對幫浦效能的影響 21-1-2 有無輔助電極穩態研究 21-1-3 有無輔助電極暫態研究 22-1-4 旅波使不同細胞粒子移動效能測試 23-1-5 實驗旅波式介電泳幫浦注意事項 23.2 全血稀釋結果 23-2-1 以生理食鹽水稀釋結果 24-2-2 以血漿稀釋結果 25-2-3 以不同樣本稀釋結果 26 4.3 介電泳幫浦流道改良 26-3-1 不同流道外型的實驗結果 26 4.4 利用旅波式介電泳分離細胞 27-4-1 定義偏移量 28-4-2 懸浮型細胞於PBS緩衝液中的偏移情形 29-4-3 貼附型細胞及白血球於培養液中的偏移情形 29-4-4 貼附型細胞黏著性對實驗結果的影響 30 五章 結論與未來展望 31 5.1 結論 31 5.2 未來展望 33 考文獻與書目 35圖表 38錄一 貼附型細胞的收集與繼代培養 84錄二 貼附型細胞培養液的調配與保存 85錄三 純化白血球濃厚液流程 86 | en |
dc.format | application/pdf | en |
dc.format.extent | 7047296 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | en |
dc.language.iso | en_US | - |
dc.subject | 旅波式介電泳幫浦 | zh-TW |
dc.subject | 輔助電極 | zh-TW |
dc.subject | 細胞分離 | zh-TW |
dc.subject | Travelling wave dielectrophoretic pump | en |
dc.subject | Assistant electrode | en |
dc.subject | Cell separation | en |
dc.title | 介電泳細胞分離與輸送的研究 | zh-TW |
dc.title | Study of the Cell Separation and Delivery Using Dielectrophoresis | en |
dc.type | thesis | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/183555/1/ntu-98-R96543036-1.pdf | - |
item.openairetype | thesis | - |
item.openairecristype | http://purl.org/coar/resource_type/c_46ec | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en_US | - |
item.fulltext | with fulltext | - |
item.grantfulltext | open | - |
顯示於: | 應用力學研究所
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