On the Immunoassay of Human Immunoglobulin using Quartz Crystal Microbalance: Experiment and Numerical Simulation
Date Issued
2008
Date
2008
Author(s)
Liao, Po-Jen
Abstract
Quartz Crystal Microbalance (QCM) biosensor has several advantages in analyzing the interaction among biomolecules, such as label free, non-destructive, highly sensitive and capable of monitoring dynamic biomolecular interaction in real time. It has been widely used as the apparatus for the biomolecule detection in the last two decades. Most of the existing works are focused merely on experiments of using QCM. In this thesis, we perform not only on experiments but also the numerical simulation based on the FEM software, COMSOL Multiphysics, to study the behavior of the antibody-antigen interaction. In these immunoassay experiments, we use the biosensor (Quartz Crystal Microbalance, QCM) to detect the specific binding reaction of the Human IgG1-Anti-Human IgG1 protein pair in physiological environments. In addition to experiments, we use the finite element analysis software, COMSOL Multiphysics, to simulate the behavior of Human IgG1 and Anti-Human IgG1 interactions. During the simulation process, we discuss the unsteady convective diffusion in fully developed laminar flow in the transport tube. The transport tube is used for transporting the analyte solution into the microchannel. The analyte concentration in the tube is strongly affected by the flow field. With the supplement of the analyte solution decreasing, the analyte concentration will be apparently decayed when length of the tube is fixed. Furthermore, the assumption of [A]surface = [A]bulk in the basic kinetic analysis is not correct because of the effect by mass transport in the liquid phase. These above-mentioned reasons cause that the apparent association rate constant and the apparent dissociation rate constant obtained by the basic kinetic analysis are not the real constants of the specific binding reaction. The apparent and the apparent cause that results obtained from the simulation and the experiment do not match. Therefore, we create a modified method to improve the basic kinetic analysis. We can obtain the calibrated and in terms of this modified method. Using the calibrated and to simulate interaction curves, we can obtain more consistent simulation results with experiments.
Subjects
Biosensor
Quartz Crystal Microbalance
FEM
Basic kinetic analysis
Human IgG1
Type
thesis
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