An Integrated Microfluid Switching Device for In-vitro Chemosensitivity Assay
Date Issued
2008
Date
2008
Author(s)
Yang, Chun-Hui
Abstract
Here we present an integrated microfluidic platform which includes two parts: flow switching module and cell culture module. This platform reduces the routine and manual work in conventional biological experiments which need to introduce numerous reagents in sequence. The fabrication of this platform dose not need the cleanroom technology which is relatively time-consuming and expensive. Pneumatic membrane valves are developed to switch the flow between different fluids by controlling the pressure. Wells of inlets are designed as the space which can temporarily store the fluids and prevent the bubble formation. The auxiliary pressure (~0.5psi) is applied from upstream to eliminate bubbles in minutes due to the air-permeability of polydimethylsiloxane (PDMS). To avoid the crosstalk between channels which introduce different fluids simultaneously, a blocking valve is added under the intersection of the channels and successfully inhibits the crosstalk. The performance of the blocking valve and the flow switching has been characterized under certain conditions. The cell culture and chemodrug screening are also demonstrated as the application of this platform.
Subjects
flow switch
pneumatic membrane valve
bubble elimination
cell culture
drug screen
chemosensitivity assay
Type
thesis
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