Detection of anti-epileptic drug phenytoin by piezoresistive microcantilever biosensor
Date Issued
2012
Date
2012
Author(s)
Lin, Lung-Yi
Abstract
Therapeutic drug monitoring is a growing issue to measure specific drugs at timed intervals in order to maintain a relatively constant concentration of the medication in the bloodstream. This study focus on “Phenytoin” which is related to cardiovascular and brain vascular diseases, and have narrow ranges of drug dosage in the bloodstream. Phenytoin acts to suppress the abnormal brain activity seen in seizure by reducing electrical conductance. By utilizing MEMS technology, a portable piezoresistive microcantilever biosensor is designed, fabricated, and integrated with a microfluidic channel system to provide immediate and efficient cares for patients at home or bedside point of care.
To develop a biosensor with label-free feature, the piezoresistive microcantilever biosensor based on nanomechanics as a transducer for drug detection. Firstly, a self-assemble molecule (SAM) has been utilized to link the gold surface on the sensing surface of a microcantilever, which induces nanomechanics of specific binding. Followed by the second layer of drug-specific antibody, the microcantilever biosensor is ready for drug detection, converting the surface stress-induced deflection into a signal of electrical resistance change. Meanwhile, by using multiple sensing microcantilevers in parallel for detection, the signal to noise ratio is increased by 189% to improve the device performance. The Phenytoin drug concentration of 10~500 μg/mL has been firstly demonstrated to ensure the measurement of drug by using this microcantilever biosensor technology.
Subjects
phenytoin
surface stress
piezoresistive
microcantilever
signal averaging
Type
thesis
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