Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Engineering / 工學院
  3. Applied Mechanics / 應用力學研究所
  4. Cytotolytic activity and characterization of cell-based Biosensor for human natural killer cells against leukemic cells
 
  • Details

Cytotolytic activity and characterization of cell-based Biosensor for human natural killer cells against leukemic cells

Date Issued
2010
Date
2010
Author(s)
Chung, Yu-Wei
URI
http://ntur.lib.ntu.edu.tw//handle/246246/249978
Abstract
Leukemia, commonly known as blood cancer, is a kind of serious diseases in which cells increase abnormally and invade adjacent tissues. High mortality rate of leukemia indicates its need of effective treatments to cure leukemia[1]. One of promising treatments is cell therapy that requires cell transplantation from healthy donors to patients. The natural killer (NK) cell therapy is chosen due mainly to its natural ability to kill tumor cells with no need of HLA match prior to transplantation. However, natural killer (NK) cells from healthy donors exhibit various levels of cytotoxicity to tumor cells. Effective investigation of the cytotoxicity of natural killer cells to tumor cells remains a great challenge. This work has developed a cell-based microfluidic device which is capable of investigating the cytotoxicity of natural killer cells against leukemic cells. The whole process of cytolytic activity was observed in real-time under the microscopic system. The lysed k562 cells (leukemic cells) were detected by PhiPhiLux (OncoImmunin, Inc.). All the E/T ratios counted in this work were in a conjugated form, which is the most specific way to count E/T ratios. It was found that the higher conjugated E/T ratio had better cytotoxicity after one hour. Morever, apparent difference in cytotoxocity of the microchannel in gap lengths of 100 μm and 400 μm was found. In a microchannel with a gap length of 100 μm, the cytotoxicity at the conjugated E/T ratio of 1,2,3,4 were 0.75, 0.8, 1, 1, respectively. In a device with a gap length of 400 μm, the cytotoxicities were 0.63, 0.33, 0.75, 0.67, respectively. The mean value of cytotoxicity over all conjugated E/T ratio was higher than 0.6. The total conjugated E/T ratios formed in three microchannels were between 2.1 and 2.3. The averaged cytotoxicities were 0.85, 0.72 and 0.63 in 100 μm, 200 μm and 400 μm channel, respectively. By comparing those three microfluidic channels, it was found that the small size channel formed the higher density of the cells and thus performed the higher cytotoxicity. Unlike the conventional cell culture experiments in dishes with large amounts of cells, this work provides a device that needs very few cells for manipulation and significantly reduces the consumption of culture medium. The microscopic system also provides an in-depth understanding of cell interaction and cytotolytic activity. This microfluidic device exhibits the capability and potential for effective investigation of natural killer cells (NK cells) against tumor cells (K562 cells).  
Subjects
leukemia
natural killer cell
leukemic cell
cell-based biosensor
cytotoxicity
conjugated E/T ratio
SDGs

[SDGs]SDG3

Type
thesis
File(s)
Loading...
Thumbnail Image
Name

ntu-99-R97543026-1.pdf

Size

23.53 KB

Format

Adobe PDF

Checksum

(MD5):e8ff03a1f0aad2073b3156515ec7be14

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science