A Mass-producible Filtration Chip for Isolation of Circulating Tumor Cells from Human Blood
Date Issued
2012
Date
2012
Author(s)
Chuang, Pei-Chen
Abstract
The detection of circulating tumor cells (CTCs) is a promising harmless in vitro diagnosis of metastasis. Cancer metastasis leads to roughly 90% of deaths of cancer patients. However, CTC detection has not been routinely used for disease management, where one reason for this is the difficulty associated with enrichment of these extremely rare cells. This work presents a microdevice to enrich CTCs from peripheral blood by a size-limiting microfilter structure. This PMMA structure is microinjection-mold and bonded onto a CNC-milled reservoir. Cells from a wide range of cancer cell lines (K562, MCF7, PC3 and Colo205) are captured on the structure, immunohistochemically stained and enumerated with fluorescent microscopy. Results show the chip is robust and ease to use. The recovery yield of cells spiked into 1mL of whole blood (diluted to 15mL) attained 70% to 80%. This microdevice should have potential to enable CTC-detection a more viable in cancer management.
Subjects
size-based
rare cell
cell separation
cell isolation
low cost
PMMA
injection molding
mass-production
Circulating tumor cells
CTCs
SDGs
Type
thesis
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