|Title:||Influence of hypothermic conditions on primary porcine hepatocyte-entrapped hollow fiber bioreactors||Authors:||Lin, Chi-Chang
|Keywords:||pH value;acetate;enzymatic hydrolysis;inhibition;particulate organic waste;kinetic models||Issue Date:||27-Feb-2005||Publisher:||Taipei:National Taiwan University Dept Biomed Engn||Source:||Biochemical Engineering Journal 29 (2006),139–148||Start page/Pages:||-||Abstract:||
Transportation of bioartificial liver (BAL) device with viable cells and higher metabolic functions are necessary. The aim of this study
is to evaluate hypothermic (4 ◦C) influence on the performance of hepatocyte-entrapped hollow fiber bioreactors, as well as the effects on
cell function with or without medium supplement. Oxygen consumptions were stable at average of 15–25mmHg per cartridge during 8 h
normothermic incubation after cold-perfusion and cold-non-perfusion. All groups showed increase in glutamic oxaloacetic transaminase
(GOT) and lactate dehydrogenase (LDH) level. Urea production and albumin synthesis were only slightly higher in cold-perfusion groups
than that of the cold-non-perfusion groups and the control. There were no significant differences in the metabolic functions of bioreactors
between each study groups during normothermic operation. Our study suggests that the use of a preliminary cold-storage step prior to normal
culture condition or clinical therapy can prolong the transportation time without changing various functions of the BAL device.
|Appears in Collections:||醫學工程學研究所|
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