|Title:||Human Monocyte Adhesion and Activation on Crystalline Polymers with Different Mophology and Wettability in Vitro
|Keywords:||surface mophology;surface wettability;monocyte;adhesion;activation||Issue Date:||2000||Source:||JOURNAL OF BIOMEDICAL MATERIALS RESEARCH||Journal Volume:||v.50||Journal Issue:||n.4||Start page/Pages:||490-498||Abstract:||
This study evaluated the effects of crystalline polyamide ( Nylon-66), poly(ethylene-co-vinyl alcohol) (PEVA), and poly( vinylidene fluoride) ( PVDF) polymers with nonporous and porous morphologies on the ability of monocytes to adhere and subsequently activate to produce IL-1 beta, IL-6, and tumor necrosis factor alpha. The results indicated monocyte adhesion and activation on a material might differ to a great extent, depending on the surface morphology and wettability. As the polymer wettability increases, the ability of monocytes to adhere increases but the ability to produce cytokines decreases. Similarly, these polymers, when prepared with porous surfaces, enhance monocyte adhesion but suppress monocyte release of cytokines. Therefore, the hydrophobic PVDF with a nonporous surface stimulates the most activity in adherent monocytes but shows the greatest inhibition of monocyte adhesion when compared with all of the other membranes. In contrast, the hydrophilic Nylon-66, which has a porous surface, is a relatively better substrate fur this work. Therefore, monocyte behavior on a biomaterial may be influenced by a specific surface property . Based on this result, we propose that monocyte adhesion is regulated by a different mechanism than monocyte activation . Consequently , the generation of cytokines by monocytes is not proportional to the number of cells adherent to the surface. (C) 2000 John Wiley & Sons, Inc.
|Appears in Collections:||醫學工程學研究所|
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