https://scholars.lib.ntu.edu.tw/handle/123456789/83300
標題: | 化學固定天然骨生長激素於可降解性生物陶瓷做為可再生性之骨替代材料 The immobilization of Chinese herbal medicine onto the surface-modified calcium hydrogenphosphate |
作者: | 林輝 Lin, Feng-Huei |
公開日期: | 31-七月-2002 | 出版社: | 臺北市:國立臺灣大學醫學工程學研究所 | 摘要: | To accelerate the healing of bone defects or to enable to heal at all, it is often necessary to fill them with suitable substance. Various artificial materials defects have been developed. Among these, calcium phosphates and bioactive glass have been proven to be biocompatibile and bioactive materials that can chemically bond with bone, and have been successfully used clinically for repair of bone defects and augmentation of osseous tissue. However, those bioceramics have only the property of osteoconduction without any osteoinduction. Many ligands have been physicochemically absorbed onto substrates to enhance cell-substrate interactions. Although it has been widely developed, it is still limited to use in long-term implantation because of short half-life period. Thus, some interfacial modification will be required for enhancing the efficacy of delivery system. These models involve the immobilization of biologically active ligands of natural and synthetic origin onto various substrates to produce an interface with stronger chemical bond between ligand and substrate. The advantage of covalently immobilizing a ligand is that a chemical bond is present to prevent ligand or medicine from desorption. In our study, two-step of chemical immobilization was performed to surface-modified calcium hydrogenphosphate powders. The first was to modify the surface of calcium hydrogen-phosphate (CHP) with coupling agent of hexanmethylene diisocyanate (HMDI). The linkage between CHP and HMDI will be characterized by FTIR. The second step was to immobilize chemically Gusuibu onto MCHP. Moreover, the sorption and desorption of Gusuibu was evaluated and quantitative analyzed by spectrophotometer and HPLC. From the results, bioceramic CHP was surface-modified by two-step of chemical immobilization. Firstly, successfully modified the surface of calcium hydrogen-phosphate (CHP) with coupling agent of hexanmethylene diisocyanate (HMDI). The first step was also activated the surface of CHP to induce primary amine terminator. The reaction of this functional group with Gusuibu was the second step. We confirmed simultaneously that Gusuibu could be immobilized chemically onto the surface of MCHP. Although some immobilized Gusuibu also released rapidly at the first 12 hours, the degree of released Gusuibu was lower than both by Gusuibu-adsorbing MCHP and Gusuibu-adsorbing CHP. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/22362 | 其他識別: | 902314B002352 | Rights: | 國立臺灣大學醫學工程學研究所 |
顯示於: | 醫學工程學研究所 |
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902314B002352.pdf | 282.89 kB | Adobe PDF | 檢視/開啟 |
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