https://scholars.lib.ntu.edu.tw/handle/123456789/83308
標題: | 光動力療法合併高溫腫瘤治療對於大腸癌細胞之影響 | 作者: | 王正一 | 關鍵字: | 光動力療法;高溫腫瘤治療;大腸癌細胞;photodynamic therapy;hyperthermia;colon cancer cells | 公開日期: | 31-七月-2003 | 出版社: | 臺北市:國立臺灣大學醫學工程學研究所 | 摘要: | 惡性腫瘤近幾年來一直是台灣地區十大死因的首位,值得注意的是結腸直腸癌排名第 三名且有逐年增加的趨勢。儘管醫學科技日益進步,但目前癌症的主要治療仍脫離不了以 手術切除、化學治療以及放射線治療等方式來破壞或抑制癌細胞增生。光動力治療是一種 新興的癌症治療方式,主要應用於治療固態癌症,其作用機轉係以雷射光源或是其它非雷 射光源激發細胞內的光感藥物,光感藥物受到光子的刺激逐步產生光化學作用,形成單相 氧分子或自由基並破壞細胞內微細結構如粒腺體,因而導致細胞死亡。高溫腫瘤治療在臨 床上由於會破壞癌細胞的細胞的膜構造而抑制其生長。兩種治療方式造成癌細胞死亡的機 制不同,將其合併在治療上有加成的效果。 本計畫探討單獨使用光動力療法及高溫處理對於大腸癌細胞的影響,研究發現光動力 療法或是高溫處理後,大腸癌細胞活性隨著時間增長而降低,LDH 隨著時間增加而增高, 利用Annexin V-FITC/PI 螢光染色觀察細胞都呈現凋亡及壞死的現象。光動力療法及高溫腫 瘤治療皆會造成細胞死亡,但是機制不盡相同,因此如何合併才能達到最好的治療效果, 有其進一步探討的必要。 Recently, malignancy is the leading cause of mortality in Taiwan, and colon cancer accounted approximately 4000 deaths each year. Photodynamic therapy (PDT) is a promising new treatment for cancer that has been recently accepted in the clinic. PDT involves the localization of a light-sensitive drug in the target tissue prior to illumination using an appropriate wavelength. Exposure of tissue containing a sufficient concentration of photosensitizer to irradiation of adequate wavelengths initiates photodynamic activation to generate single oxygen free radicals and other reactive species, causing cellular damage and tissue necrosis. It is a procedure having the potential to selectively destroy malignant tissues without causing serious damage to the adjacent normal structures if light dosimetry can be delivered with care. This study relates to studies on modes of phtotoxicity after incubation of 5-aminolevulinic acid (5-ALA) on colon cancer cells and compared with hyperthermia treated. The viability results show that the survivals of the colon cancer cells were decreased after PDT or hyperthermia alone as incubation time increased. The Annexin V-FITC/PI assay shows that the cells were apoptosis after PDT or hyperthermia treatment alone. Although PDT and hyperthermia both cause cell apoptosis, the mechanism are different. How to combine these two treatments and the sequence are need to study more. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/22371 | 其他識別: | 912213E002118 | Rights: | 國立臺灣大學醫學工程學研究所 |
顯示於: | 醫學工程學研究所 |
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912213E002118.pdf | 57.03 kB | Adobe PDF | 檢視/開啟 |
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