https://scholars.lib.ntu.edu.tw/handle/123456789/83684
Title: | Chondrogenesis From Immortalized Human Mesenchymal Stem Cells: Comparison Between Collagen Gel and Pellet Culture Methods | Authors: | Chang, Chih-Hung Lin, Hsin-Yi Fang, Hsu-Wei Loo, Siow-Tung Hung, Shih-Chieh Ho, Yi-Ching Chen, Chia-Chun Lin, Feng-Huei Liu, Hwa-Chang |
Keywords: | human mesenchymal stem cells;cartilage tissue repair;collagen scaffold;biodegradable materials;chondrogenesis | Issue Date: | 2008 | Journal Volume: | v.32 | Journal Issue: | n.7 | Start page/Pages: | 561-566 | Source: | Artificial Organs | Abstract: | Human mesenchymal stem cells (hMSCs) can differentiate into cells of connective tissue lineages, including cartilage. To overcome the limiting autogenous chondrocyte populations available in cartilage repair, various methods have been developed to maximize chondrogenesis of hMSCs in vitro, most of which use cells derived from primary culture. In this study, we compared chondrogenesis of immortalized hMSCs embedded in collagen gel to those grown in pellet culture. The hMSCs in collagen scaffolds expressed more glycosaminoglycan than those in pellet culture. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis demonstrated that the expression of genes encoding sox-9, aggrecan, and types I and II collagen increased in pellet culture over time. However, in the collagen cultures, only type II collagen and aggrecan expression increased over time, whereas sox-9 expression remained unchanged and type I collagen expression decreased. These results indicate that the immortalized hMSC line is a promising tool for further in vitro chondrogenic studies. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/128043 https://www.scopus.com/inward/record.uri?eid=2-s2.0-47349100935&doi=10.1111%2fj.1525-1594.2008.00575.x&partnerID=40&md5=6f100ca16dd10a10fcfcbe5fc91860e4 |
ISSN: | 0160564X |
Appears in Collections: | 醫學工程學研究所 |
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