|Title:||Immobilization of l-lysine on dense and porous poly(vinylidene fluoride) surfaces for neuron culture||Authors:||Young, Tai-Horng
|Keywords:||Poly(vinylidene fluoride);Porous;Membrane;Immobilization;l-lysine||Issue Date:||2008||Source:||Desalination||Journal Volume:||v.234||Journal Issue:||n.1-3||Start page/Pages:||134-143||Abstract:||
Microporous poly(vinylidene fluoride) (PVDF) membranes with either dense or porous Surface were prepared by isothermal immersion-precipitation of a casting solution in coagulation baths of different strengths. Onto the membrane surface, an amino acid (l-lysine) was immobilized by a dual-step chemical process. First, the membrane was grafted with poly( acrylic acid) (PAA) by means of plasma-induced free radical polymerization. Then, l- lysine was covalently bonded to the as-grafted PAA chains with the aid of a water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl)- carbodiimide hydrochloride (EDC). The highest attainable graft yield of PAA on PVDF membrane reached up to 0.64 mg/cm(2). For immobilization of l- lysine on the membrane, the yields were found to depend on factors, Such as concentration of EDC, activation time, and pH value. The maximal attainable immobilization yield was 0.65 mu g/cm(2). Furthermore, pheochromocytoma (PC12) cells were cultured on l-lysine/PAA/ PVDF membranes. It was found that both the amount of l- lysine on the membrane and the Surface structure had a marked influence on the cell activity. Thus, the present results could be useful for the development of strategies to promote the re-growth and regeneration Of tissue in the nervous system.
|Appears in Collections:||醫學工程學研究所|
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