https://scholars.lib.ntu.edu.tw/handle/123456789/83898
標題: | Pressure Effects on the Growth of Human Scar Fibroblasts | 作者: | Chang, Liang-Wey Deng, Win-Ping Yeong, Eng-Kean Wu, Ching-Yuan Yeh, Shih-Wei 章良渭 鄧文炳 楊永健 吳慶源 葉士緯 |
公開日期: | 2008 | 卷: | v.29 | 期: | n.5 | 起(迄)頁: | 835-841 | 來源出版物: | Journal of Burn Care & Research | 摘要: | Although pressure therapy is the mainstay of treatment for hypertrophic scars, its actual mechanism remains unknown. An in vitro study was designed to investigate the effects of positive pressure on the growth of human scar-derived fibroblasts through its transforming growth factor beta 1 ( TGF-beta 1) secretion. A pneumatic pressure system connecting to a cell culture chamber was designed. Six-well cultured plates with fibroblasts implanted were treated with different pressure settings. Cells were treated with constant pressure 20 mm Hg above atmosphere pressure ( group A n = 18) or with 40 mm Hg above atmosphere pressure (group B n = 18 ) daily for nine successive days. Cells without pressure were treated as the control study (group C n = 6). Each experimental group was divided into daily pressure applied at 24 hours (n = 6), 18 hours (n = 6), and 12 hours (n = 6). Cell counting was performed oil the 2nd, 4th, 7th, 9th, 11 th, and 14th day after implantation. On day 4, the concentration of transforming growth factor beta 1 was measured, and cell doubling time was calculated. Compared with the control group, there was a significant decrease in cell count and the concentration in the 18-hour and 24-hour 20 mm Hg or 40 mm Hg pressure treated group. The cell doubling time was significantly increased in the 24-hour 20 mm Hg or 40 mm Hg pressure treated groups, and the 18-hour 40 mm Hg pressure treated group. (P < .05) Pressure inhibits the growth and activity of human scar fibroblasts, and a higher pressure application can shorten the daily application period. There should be an optimal pressure level corresponding to a daily application period to achieve the most effective results on pressure therapy for scars. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/128177 |
顯示於: | 醫學工程學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。