|Title:||Neurons Cultured on Gan and Is Associated with Synapsin I and Map2 Expression||Authors:||Chen, Chi-Ruei
|Keywords:||gallium nitride (GaN);cerebellar granule neurons;LDH;synapsin I||Issue Date:||2008||Source:||Biomedical Engineering Applications Basis and Communications||Start page/Pages:||75-82||Abstract:||
In this work, the behaviors of cerebellar granule neurons prepared from 7 -day-old Wistar rats on GaN, GaAs, and silicon were investigated. We believe that this is the first time that the GaN has been used as a substrate for neuron cultures to examine its effect on cell response in vitro. The GaN surface structure and its relationship with cells were examined by scanning electron microscopy ( SEM), immunofluorescence lactate dehydrogenase ( LDH). Compared with silicon used for most neural chips, neurons seeded on GaN were able to form an extensive neuritic network and expressed very high levels of synapsin I coincident with the neurite outgrowth. LDH assay indicated that GaN improve neuron survival better than silicon and GaAs. Between in seven-day and day 15-cultured neurons, these results are consistent with the influence of GaN, in the regulation of neuronal adhesion, neuritic plasticity and survival, within in vitro. The favorable biocompatibility characteristics of GaN can be used to measure electric signals from networks of neuronal cells in culture to make it a possible candidate for use in a microelectrode array.
|Appears in Collections:||醫學工程學研究所|
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