|Title:||Immobilization of L-Lysine on Microporous Pvdf Membranes for Neuron Culture||Authors:||YOUNG, TAI-HORNG
|Keywords:||Poly(vinylidene fluoride);membrane;immobilization;L- lysine;neuron;culture||Issue Date:||2009||Source:||Journal of Biomaterials Science Polymer Edition||Start page/Pages:||703-720||Abstract:||
Microporous poly(vinylidene fluoride) (PVDF) membranes with dense or porous surface were prepared by immersion precipitation of PVDF/TEP solutions in coagulation baths containing different amounts of water. Onto the membrane surface, poly(glycidyl methacrylate) (PGMA) was grafted by plasma-induced free radical polymerization. Then, L-lysine was covalently bonded to the as-grafted PGMA through ring- opening reactions between epoxide and amine to form amino alcohol. The highest attainable graft density of PGMA on a PVDF membrane was 0.293 mg/cm(2). This was obtained when the reaction was carried out on a porous surface under an optimized reaction condition. For immobilization of L-lysine , the yield was found to depend on the reaction temperature and L-lysine concentration. The maximal yield was 0.226 mg/ cm(2), a value considerably higher than reported in the literature using other immobilization methods. Furthermore , neurons were cultured on L-lysine-immobilized PVDF membranes . The results indicated that these membrane surfaces were suited to the growth of neurons, with a MTT value higher than that of the standard culture dish .
|Appears in Collections:||醫學工程學研究所|
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