Imaging morphodynamics of human blood cells in vivo with video-rate third harmonic generation microscopy
Resource
Biomedical Optics Express,3,2860-2865
Journal
Biomedical Optics Express
Pages
2860
Date Issued
2012-11
Date
2012-11
Author(s)
Chen, C.-K.
Liu, T.-M.
Abstract
With a video-rate third harmonic generation (THG) microscopy system, we imaged the micro-circulation beneath the human skin without labeling. Not only the speed of circulation but also the morpho-hydrodynamics of blood cells can be analyzed. Lacking of nuclei, red blood cells (RBCs) shows typical parachute-like and hollow-core morphology under THG microscopy. Quite different from RBCs, every now and then, round and granule rich blood cells with strong THG contrast appear in circulation. The corresponding volume densities in blood, evaluated from their frequencies of appearance and the velocity of circulation, fall within the physiological range of human white blood cell counts.
Subjects
Three-dimensional microscopy
Diagnostic applications of nonlinear optics
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