https://scholars.lib.ntu.edu.tw/handle/123456789/85625
標題: | Assessment of heavy metal bioavailability in contaminated sediments and soils using green fluorescent protein-based bacterial biosensors | 作者: | Liao, Hsiu-Chuan Chien, Ming-Te Tseng, Yuen-Yi Ou, Kun-Lin |
關鍵字: | bandpass filter;diplexer;microstrip filter;stepped-impedance resonator (SIR);transmission zero;Green fluorescent protein;Bacterial biosensor;Bioavailable heavy metals;Contaminated sediments and soils | 公開日期: | 29-九月-2006 | 出版社: | Taipei:National Taiwan University | 卷: | 142 | 期: | 2006 | 起(迄)頁: | - | 來源出版物: | Environmental Pollution | 摘要: | A green fluorescent protein (GFP)-based bacterial biosensor Escherichia coli DH5a (pVLCD1) was developed based on the expression of gfp under the control of the cad promoter and the cadC gene of Staphylococcus aureus plasmid pI258. DH5a (pVLCD1) mainly responded to Cd(II), Pb(II), and Sb(III), the lowest detectable concentrations being 0.1 nmol L1, 10 nmolL1, and 0.1 nmol L1, respectively, with 2 h exposure. The biosensor was field-tested to measure the relative bioavailability of the heavy metals in contaminated sediments and soil samples. The results showed that the majority of heavy metals remained adsorbed to soil particles: Cd(II)/Pb(II) was only partially available to the biosensor in soilewater extracts. Our results demonstrate that the GFP-based bacterial biosensor is useful and applicable in determining the bioavailability of heavy metals with high sensitivity in contaminated sediment and soil samples and suggests a potential for its inexpensive application in environmentally relevant sample tests. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/2006111501254683 | 其他識別: | 246246/2006111501254683 |
顯示於: | 生物環境系統工程學系 |
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