DC 欄位 | 值 | 語言 |
dc.contributor.author | 徐濟泰 | zh_TW |
dc.creator | 徐濟泰 | en |
dc.date | 2002 | - |
dc.date.accessioned | 2006-07-26 | - |
dc.date.accessioned | 2018-06-29T04:16:31Z | - |
dc.date.available | 2006-07-26 | - |
dc.date.available | 2018-06-29T04:16:31Z | - |
dc.date.issued | 2002 | - |
dc.identifier | 902313B002313 | zh_TW |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/16449 | - |
dc.description.abstract | 本試驗以瘤胃中之纖維分解菌F.succinogenes S85 為纖維素分解酵素及纖維素結合蛋白質(CBP)來源,並利用R.. albus
7 之XynC 基因產物為聚木糖分解酵素(xylanase)來源,測試添加纖維素結合蛋白質後之酵素活性表現。XynC 基因之產物
約為62 kDa,且具有高活性之聚木糖分解酵素活性。來自F.. succinogenes S85 之纖維素分解酵素經離子交換法及膠體過濾法純化後可得約120 及65 kDa 兩個主要蛋白質,且經活性染色確定其酵素活性。CBP利用含10%纖維二糖之磷酸鹽緩衝液配合1%CHAPS 進行回收。XynC 基因產物之聚木糖分解酵素與純化後之纖維素分解酵素,在加入CBP 後可有效提升其酵素作用
之特異性4 至10 倍,且發現CBP 可作用於不同菌株而來之分解酵素,提升酵素之效力。 | zh_TW |
dc.description.abstract | Attempts were made to examine enzymes
activity after addition cellulose binding
protein (CBP). Cellulase and CBP were
purified from rumen cellulytic bacterium F..
succinogenes S85, and the gene encoding
xylanase C (XynC) of rumen cellulytic
bacterium R. albus 7 was be the source of
xylanase. The gene encoded xylanase was 62
kDa, and detected high xylanase activity.
Cellulase from F.. succinogenes S85, after
passing ion exchange and gel filtration
columns, present two protein peaks with
cellulase activity, and the molecular weight
was approximately 120 and 65 kDa,
respectively. CBP was eluted with sodium
phosphate buffer containing 10% cellobiose
and 1% CHAPS. Enzymes activity were
increasing from four to ten times after
addition of CBP. The result showed that CBP
can effect on both cellulytic and
hemicellulytic enzyme from different bacteria
species and improve their enzyme activity. | en |
dc.format | application/pdf | en |
dc.format.extent | 202600 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | zh_TW |
dc.language.iso | zh_TW | - |
dc.publisher | 臺北市:國立臺灣大學動物科學技術學系暨研究所 | zh_TW |
dc.rights | 國立臺灣大學動物科學技術學系暨研究所 | zh_TW |
dc.subject | 纖維素分解酵素 | zh_TW |
dc.subject | 聚木糖分解酵素 | zh_TW |
dc.subject | 纖維素結合蛋白質 | zh_TW |
dc.subject | 酵素活性 | zh_TW |
dc.subject | cellulase | en |
dc.subject | xylanase | en |
dc.subject | cellulose | en |
dc.subject | binding protein | en |
dc.subject | enzyme activity | en |
dc.title | 利用生物技術大量產製瘤胃細菌纖維分解酵素複合體 | zh_TW |
dc.type | report | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/16449/1/902313B002313.pdf | - |
dc.coverage | 計畫年度:90;起迄日期:2001-08-01/2002-07-31 | zh_TW |
item.fulltext | with fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | report | - |
item.languageiso639-1 | zh_TW | - |
item.openairecristype | http://purl.org/coar/resource_type/c_93fc | - |
item.grantfulltext | open | - |
crisitem.author.dept | Animal Science and Technology | - |
crisitem.author.orcid | 0000-0002-7701-3163 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
顯示於: | 動物科學技術學系
|