https://scholars.lib.ntu.edu.tw/handle/123456789/87844
標題: | 建立商業纖維分解酵素於反芻動物營養應用價值之評估方法 | 作者: | 徐濟泰 | 關鍵字: | 苜蓿;狼尾草;玉米青貯;商業纖維分解酵素;試管乾物質消化率;alfalfa;napier;corn silage;commercial fibrolytic enzymes;in vitro dry matter digestibility | 公開日期: | 2003 | 出版社: | 臺北市:國立臺灣大學動物科學技術學系暨研究所 | 摘要: | 本研究計畫,以各兩種不同成熟度的禾本科狼尾草與豆科苜蓿以及一種玉米青貯等五種芻料當為測試纖維分解對象,再收集自餵飼高芻料或高精料乾乳牛瘤胃液,分別進行四種市售商業纖維分解酵素產品單獨或不同組合之試管消化試驗,同時分析各酵素產品之不溶性纖維素與半纖維素分解酵素、α-L-rabinofuranosidase、feruloyl esterase 活性,以及分析在試管內與瘤胃液組合後之cellulase、xylanase、α-L- arabinofuranosidase、feruloyl esterase 個別酵素總活性以及試管消化率。Hemicell 是四種市售商業酵素中ellulase、hemicellulase 與ferulyl esterase 活性均最強。Selfeed 與Bio-act 兩種商品酵素活性,以cellulase 與hemicellulase 為主。VP-100 則是以hemicellulase 與 α-L-arabinofuranosidase 為主。置於餵飼高芻料乾乳牛瘤胃液中,四種市售商業酵素均能顯著提昇cellulase 與feruloyl esterase 活性表現,只有Hemicell 與VP-100 可顯著提昇hemicellulase 活性表現,只有VP-100 可顯著提昇 α-L-arabinofuranosidase 活性表現。四種市售商業纖維分解酵素均可顯著提昇未開花狼尾草與玉米青貯的消化率,只有VP-100 顯著提昇開花狼尾草48 小時試管乾物質消化率,而VP-100、Selfeed、Bio-act 均顯著提昇開花苜蓿消化率。置於餵飼高精料乾乳牛瘤胃液中,四種市售商業纖維分解酵素均可顯著 提昇未開花苜蓿的消化率。Bio-act 除外的其他三種市售商業纖維分解酵素均可顯著提昇未開花狼尾草的消化率。只有Hemicell 與Selfeed 顯著提升開花狼尾草與開花苜蓿在高精料瘤胃液的消化率,可能是因為Hemicell 與Selfeed適合作用的pH 是如同餵飼高精料的偏酸性的條件,而VP-100 可能需要高pH值,所以在高精料瘤胃液中無法顯著改善開花的老化牧草消化率。高精料瘤胃液中,再進行玉米青貯試管消化率測定,pH 偏酸性的問題更嚴重,四種市售商業纖維分解酵素均無法提升其消化率。 Ruminal fluids from dry dairy cows fed high forage (70% forage) or high concentrate (70% concentrate) diets were used to represent two ruminal conditions for testing cellulase、xylanase、α-L- arabinofuranosidase、feruloyl esterase activities of four kinds of commercial fibrolytic enzyme products and their potential to improve in vitro dry matter digestibility of pre-bloom and blooming alfalfa, pre-bloom and blooming napier grass and corn silage. Hemicell showed the highest cellulase、hemicellulase and ferulyl esterase activities among four tested cormmercial enzyme products。Selfeed and Bio-act mainly showed higher cellulase and hemicellulase activities. VP-100 had higher hemicellulase and α-L-arabinofuranosidase activities. Tested in the ruminal fluid from dry cows fed high forage diet, all four commercial enzyme products increased cellulase and feruloyl esterase activities. Hemicell and VP-100 significantly increased hemicellulase activity. Only VP-100 significantly increased α-L-arabinofuranosidase activity. All four commercial enzyme products improved the 48-hour in vitro dry matter digestibility of pre-bloom napier and corn silage. Only VP-100 improved the 48-hour in vitro dry matter digestibility of blooming napier. VP-100, Selfeed, and Bio-act all improved the 48-hour in vitro dry matter digestibility of blooming alfalfa. Tested in the ruminal fluid from dry cows fed high concentrate diet, all four commercial enzyme products improved the 48-hour in vitro dry matter digestibility of pre-bloom alfalfa. Except of Bio-act, the other three commercial enzyme products improved the 48-hour in vitro dry matter digestibility of pre-bloom napier. Only Hemicell and Selfeed significantly improved blooming napier and blooming alfalafa. Maybe fibrolytic enzymes activity were more suitable to function at lower pH range like the high concentrate fed ruminal fluid. On the contrary, VP-100 may require higher pH condition like the high forage fed ruminal fluid. This may be able to explain why only VP-100 can improved the blooming napier digestibility in the high forage fed ruminal fluid, and in stead Hemicell and Selfeed improved blooming napier digestibility in the high concentrate fed ruminal fluid. The corn grain within the corn silage may cause worse pH declining in the high concentrate fed ruminal fluid which rendered no opportunity for any commercial enzyme products to improve the digestibility of corn silage in the high concentrate fed ruminal fluid. |
URI: | http://ntur.lib.ntu.edu.tw//handle/246246/16455 | 其他識別: | 912313B002383 | Rights: | 國立臺灣大學動物科學技術學系暨研究所 |
顯示於: | 動物科學技術學系 |
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912313B002383.pdf | 228.12 kB | Adobe PDF | 檢視/開啟 |
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