|Title:||Development and characterization of a specific anti-Caveolin-1 antibody for Caveolin-1 functional study in human, goat and mouse||Authors:||Ke, Meng-Wei
Cheng, Winston Teng-Kuei
|Issue Date:||2007||Journal Volume:||20||Journal Issue:||6||Start page/Pages:||856-865||Source:||Asian-Australasian Journal of Animal Sciences||Abstract:||
Caveolin-1 of the caveolin family of proteins regulates mammary gland development and has been shown to play a contradictory role in breast tumor progression. A specific anti-Caveolin-1 antibody will be useful for functional study of Caveolin-1 in different tissues. In this study, we generated a rabbit polyclonal antibody that specifically recognizes the N-terminal amino acids 50-65 of Caveolin-1. This polyclonal antibody specifically reacted with Caveolin-1 extracted from cells of different species, including human epithelial A431 cells, goat primary mammary epithelial cells and mice fibroblast NIH 3T3 cells, by Western blotting. Endogenous Caveolin-l protein expressing in cells and normal human tissues was detected by this polyclonal antibody using immunocytofluorescent and immunohistochemical staining, respectively. Furthermore, an apparent decrease in Caveolin-1 expression in tumorous breast and colon tissues was detected by this polyclonal antibody. In conclusion, we have identified amino acids 50-65 of Caveolin-1, which contains an epitope that is specific to Caveolin-l and is conserved in the human, goat and mouse. In future, this anti-Caveolin-1 antibody can be used to examine the progression of breast and colon cancers and to study functions of Caveolin-1 in human, goat and mouse cells.
|DOI:||10.5713/ajas.2007.856||metadata.dc.subject.other:||Capra hircus; Mus; Oryctolagus cuniculus
|Appears in Collections:||動物科學技術學系|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.