https://scholars.lib.ntu.edu.tw/handle/123456789/89026
標題: | Development and characterization of a specific anti-Caveolin-1 antibody for Caveolin-1 functional study in human, goat and mouse | 作者: | Ke, Meng-Wei Jiang, Yan-Nian Li, Yi-Hung Tseng, Ting-Yu Kung, Ming-Shung CHIUN-SHENG HUANG Cheng, Winston Teng-Kuei Hsu, Jih-Tay Ju, Yu-Ten |
公開日期: | 2007 | 卷: | 20 | 期: | 6 | 起(迄)頁: | 856-865 | 來源出版物: | Asian-Australasian Journal of Animal Sciences | 摘要: | Caveolin-1 of the caveolin family of proteins regulates mammary gland development and has been shown to play a contradictory role in breast tumor progression. A specific anti-Caveolin-1 antibody will be useful for functional study of Caveolin-1 in different tissues. In this study, we generated a rabbit polyclonal antibody that specifically recognizes the N-terminal amino acids 50-65 of Caveolin-1. This polyclonal antibody specifically reacted with Caveolin-1 extracted from cells of different species, including human epithelial A431 cells, goat primary mammary epithelial cells and mice fibroblast NIH 3T3 cells, by Western blotting. Endogenous Caveolin-l protein expressing in cells and normal human tissues was detected by this polyclonal antibody using immunocytofluorescent and immunohistochemical staining, respectively. Furthermore, an apparent decrease in Caveolin-1 expression in tumorous breast and colon tissues was detected by this polyclonal antibody. In conclusion, we have identified amino acids 50-65 of Caveolin-1, which contains an epitope that is specific to Caveolin-l and is conserved in the human, goat and mouse. In future, this anti-Caveolin-1 antibody can be used to examine the progression of breast and colon cancers and to study functions of Caveolin-1 in human, goat and mouse cells. |
URI: | http://www.scopus.com/inward/record.url?eid=2-s2.0-34249911216&partnerID=MN8TOARS http://scholars.lib.ntu.edu.tw/handle/123456789/329085 http://ntur.lib.ntu.edu.tw/bitstream/246246/238490/-1/05.pdf |
ISSN: | 1011-2367 | DOI: | 10.5713/ajas.2007.856 | SDG/關鍵字: | Capra hircus; Mus; Oryctolagus cuniculus |
顯示於: | 動物科學技術學系 |
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