Preparation and properties of bio-compatible magnetic Fe3O4 nanoparticles
Journal
Journal of Magnetism and Magnetic Materials
Journal Volume
304
Journal Issue
1
Pages
e415-e417
Date Issued
2006
Date
2006
Author(s)
Huang, Pung-Ling
Chana, H.T.
Huang, P.L.
Chan, T.S.
Huang, C.Y.
Yang, S.Y.
Horng, H.E.
Abstract
In this work, we study the preparation and properties of bio-compatible magnetic nanoparticles for immunoassay and DNA detection. The magnetite (Fe3O4) nanoparticles were prepared by a chemical co-precipitation method and dextran was selected as the surfactant to suspend the nanoparticles. Suspended particles associated with avidin followed by biotin were qualitatively analyzed by enzyme-linked immunosorbent assay (ELISA) method. We found further the ethylenediamine blocked activated residual groups efficiently, hence enhancing the attachment of biotin for probing the avidin. © 2006 Elsevier B.V. All rights reserved.
Subjects
Avidin; Bio-compatible nanoparticles; Biotinylated alkaline phosphatase; Hybridization
Type
journal article
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