德國蜚蠊生物時鐘掩蓋機制探討(3/3)
Date Issued
2004
Date
2004
Author(s)
DOI
922313B002058
Abstract
The locomotor circadian rhythm was
masked by the development of ovaries in
female German cockroach (Blattella
germanica). This study explores the
masking mechanisms of the biological clock
in cockroach. Firstly, we proceed the
detection of Electroantennogram (EAG) as
the second external indicator of circadian
clock. We realize the localization of
masking mechanism of biological clock
based on EAG results. Since the masking
phenomenon is result from physiological
activity of reproduction, we choose the
mating activity as an other indicator to study
the masking effect.
Secondly, Blattella germanica and B.
bisignata are close-related species, but they
show huge differences in the expression
of circadian rhythm s before mating. The
comparative study of the expression of
circadian rhythm between two species, is a
good theme in the investigation of ecological
significance of circadian rhythm.
Comparing the DNA sequences of per gene
between B. germanica and B. bisignata, we
understand which factor influences the
2
expression of circadian clock based on the
molecular evolution of per gene.
Finally, in order to understand the effect
of reproductive factor on the output pathway
and masking the circadian rhythm of
locomotion, termite are used to investigate
the relationship between reproduction and
circadian clock in non-cockroach species.
We compare the expression of three clock
gene (per, pdf and corazonin) in individual of
different casts at different time point. This
result shows the locations where these clock
genes the interaction takes place between the
expression and transmission pathway of time
signal for locomotion. Based on the above
research, the masking mechanism are
understood clearly.
Subjects
masking effect
locomotor
behavior
behavior
circadian rhythm
period gene
Electroretinogram
Blattella germanica
Blattella
bisignata
bisignata
mating activity
DNA
sequence
sequence
termite
PDF
Corazonin
Publisher
臺北市：國立臺灣大學昆蟲學系暨研究所
Type
report
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