台灣—捷克衛生害蟲生殖調控機制合作計劃：德國蜚蠊雄性生殖副腺對雌成蟲繁殖及活動行為日週律動的影響(1/3)
Date Issued
2004
Date
2004
Author(s)
DOI
922313B002069
Abstract
The German cockroach is a cosmopolitan household species, which is closely
associated with our daily life. In this project, we investigate the function of accessory
glands on reproductive performances and locomotor circadian rhythm of the German
cockroach. The basic function of the accessory glands of male insects is to provide
materials for the production of the spermatophore. In females, the accessory glands are
important in the formation of ootheca and secretion of antibacterial proteins. Therefore, it
is of great importance to study the accessory glands for understanding the mating system
of the pest and consequently making contribution to further development of pest control.
Firstly, we will observe and describe the morphology of the accessory glands for both
sexes of the German cockroach. The relationships between accessory gland secretion and
ootheca formation, egg number, survivorship of offspring and endocrine secretion (e.g.
JH, ecdysone) for females, and spermatophore formation, time of copulation and
survivorship of offspring for males will be examined. The daily secretion rhythm of the
accessory glands will be investigated to reveal its circadian rhythm. In addition, the
effects of male accessory gland secretion on female reproductive performances (e.g.
re-mating rate, egg number, survivorship of offspring) and competition with other males’
sperms will be our focal point of research. In the last year of this project, we will conduct
the bioassay for specific proteins of the accessory glands, including antibacterial analysis,
reproductive performances and the effects on locomotor circadian rhythm of females. We
will also purify the proteins and then, analyze and sequence the amino acids. Using the
antibody of specific protein, we will be able to trace the localization of the specific
protein during and after the process of copulation. We expect to understand more about
the mating system of the German cockroach through the proposed studies. Furthermore,
the effects of accessory glands from both male and female on reproductive performances
and locomotor circadian rhythm will be elucidated.
Subjects
Blattella germanica
Culex pipiens
accessory gland protein
locomotion
circadian rhythm
Publisher
臺北市：國立臺灣大學昆蟲學系暨研究所
Type
report
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