Repository logo
  • English
  • 中文
Log In
Have you forgotten your password?
  1. Home
  2. College of Bioresources and Agriculture / 生物資源暨農學院
  3. Plant Pathology and Microbiology / 植物病理與微生物學系
  4. Identification and characterization of the CRK1 gene in Cryptococcus neoformans
 
  • Details

Identification and characterization of the CRK1 gene in Cryptococcus neoformans

Date Issued
2008
Date
2008
Author(s)
Liu, Kuang-Hung
URI
http://ntur.lib.ntu.edu.tw//handle/246246/181938
Abstract
Cryptococcus neoformans is a heterothallic basidiomycete and grows vegetatively as yeast. Under nitrogen limitation conditions, strains of opposite mating types, MATa and MATα, produce conjugation tubes and fuse to form sexual dikaryotic filaments with clamp connections. Our previous studies demonstrated that C. neoformans Cwc1 and Cwc2 are two central photoregulators which form complex to inhibit the production of sexual filaments upon light stimulation. To reveal the detailed regulatory mechanisms, Agrobacterium-mediated insertional mutagenesis screen was conducted to identify the components interacting with or functioning downstream of the Cwc1/Cwc2 complex in the pathway. Upon light irradiation, T-DNA mutants restoring different extents of filamentation under the CWC1 overexpression background were identified. In this study, one suppressor mutant, DJ22, was characterized and T-DNA was found to integrate at the C. neoformans CRK1 gene, a homologue of Saccharomyces cerevisiae IME2 and Ustilago maydis crk1. Ime2 is a meiosis-specific gene with the conserved Ser/Thr kinase domain and the significant TXY dual phosphorylation site. Consistent with the findings of other suppressors in our screen, C. neoformans Crk1 played negative role in the mating process. Mating efficiency was increased in the crk1 mutants. Dikaryotic filaments, basidia, and basidiospores produced earlier in the crosses involved the crk1 mutants. Elevation of the CRK1 mRNA level inhibited sexual differentiation. On the other hand, monokaryotic fruiting was defective both in the MATα crk1 mutant and CRK1 overexpression strains. Our study demonstrated that mating process was negatively regulated by the CRK1 gene in C. neoformans.
Subjects
Cryptococcus neoformans
File(s)
Loading...
Thumbnail Image
Name

ntu-97-R94633017-1.pdf

Size

23.53 KB

Format

Adobe PDF

Checksum

(MD5):09aec82f904a6094c1a826d872f50408

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

總館學科館員 (Main Library)
醫學圖書館學科館員 (Medical Library)
社會科學院辜振甫紀念圖書館學科館員 (Social Sciences Library)

開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

  • 請確認所上傳的全文是原創的內容,若該文件包含部分內容的版權非匯入者所有,或由第三方贊助與合作完成,請確認該版權所有者及第三方同意提供此授權。
    Please represent that the submission is your original work, and that you have the right to grant the rights to upload.
  • 若欲上傳已出版的全文電子檔,可使用Open policy finder網站查詢,以確認出版單位之版權政策。
    Please use Open policy finder to find a summary of permissions that are normally given as part of each publisher's copyright transfer agreement.
  • 網站簡介 (Quickstart Guide)
  • 使用手冊 (Instruction Manual)
  • 線上預約服務 (Booking Service)
  • 方案一:臺灣大學計算機中心帳號登入
    (With C&INC Email Account)
  • 方案二:ORCID帳號登入 (With ORCID)
  • 方案一:定期更新ORCID者,以ID匯入 (Search for identifier (ORCID))
  • 方案二:自行建檔 (Default mode Submission)
  • 方案三:學科館員協助匯入 (Email worklist to subject librarians)

Built with DSpace-CRIS software - Extension maintained and optimized by 4Science