DC 欄位 | 值 | 語言 |
dc.contributor | 國立臺灣大學植物病理與微生物學系暨研究所 | zh_TW |
dc.contributor.author | 陳昭瑩 | zh_TW |
dc.creator | 陳昭瑩 | - |
dc.date | 1998 | zh_TW |
dc.date.accessioned | 2006-07-26T01:05:03Z | - |
dc.date.accessioned | 2018-06-29T07:10:33Z | - |
dc.date.available | 2006-07-26T01:05:03Z | - |
dc.date.available | 2018-06-29T07:10:33Z | - |
dc.date.issued | 1998 | - |
dc.identifier | 872313B002043 | zh_TW |
dc.identifier.uri | http://ntur.lib.ntu.edu.tw//handle/246246/17658 | - |
dc.description.abstract | 抽取百合葉片全RNA進行圓點斑
跡雜合分析,以大麥、燕麥及菜豆抗
病相關基因作為探針,在水楊酸處理
的在百合葉片中可偵測到類似防禦基
因表現加強的情形。與大麥PR-4, 14-
3-3, chalcone synthase 及燕麥tlp 類似
的基因可能存在於百合中,且可能為
水楊酸所誘導表現。為進行北方雜合
分析,首先以PCR 法釣取百合18S
rDNA,並完成分子選殖及序列的確
認。續以RT-PCR 自百合葉片全RNA
中釣取化學物質誘導性防禦基因的
cDNA 片段。由已知幾丁質分解酵素基
因及大麥PR-4,燕麥tlp 核酸序列設計
引子,進行RT-PCR,釣取百合有抑菌
功能的基因cDNA 片段。在大麥PR-4
引子的RT-PCR 反應中獲得了與番茄
低溫表現的cysteine proteinase 有32%
相似度的cDNA 片段,可能與百合的
防禦反應有關。 | zh_TW |
dc.description.abstract | In the dot blot analysis with total
RNA of lily leaves treated with salicylic
acid (SA), the barley PR-4, 14-3-3,
chalcone synthase and oat tlp gene
probes detected stronger signals,
indicating that related genes may present
in lily and are SA inducible. In order to
perform northern hybridization analysis,
the 18S rDNA of lily was cloned and
sequenced after PCR amplification. In
order to clone the cDNA fragments of
defense genes of lily in response to the
induction of chemicals, RT-PCR was
perfomed using primers designed
according to the sequences of chitinase,
barley PR-4, and oat tlp genes. A cDNA
fragment with similar size to the
prediction was amplified. The amino
acid sequence showed 32% similarity to
2
that of cysteine proteinase of tomato, a
protein expressed in cold temperature.
This cDNA fragment was presumed
related to the defense reaction of lily. | en |
dc.format | application/pdf | zh_TW |
dc.format.extent | 39168 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language | zh-TW | zh_TW |
dc.language.iso | zh_TW | - |
dc.publisher | 臺北市:國立臺灣大學植物病理與微生物學系暨研究所 | zh_TW |
dc.rights | 國立臺灣大學植物病理與微生物學系暨研究所 | zh_TW |
dc.title | 百合抗病性防禦基因CDNA選殖及表現分析 | zh_TW |
dc.type | other | en |
dc.identifier.uri.fulltext | http://ntur.lib.ntu.edu.tw/bitstream/246246/17658/1/872313B002043.pdf | - |
dc.coverage | 計畫年度:87;起迄日期:1997-08-01/1998-07-31 | zh_TW |
item.fulltext | with fulltext | - |
item.cerifentitytype | Products | - |
item.openairetype | other | - |
item.languageiso639-1 | zh_TW | - |
item.openairecristype | http://purl.org/coar/resource_type/c_1843 | - |
item.grantfulltext | open | - |
crisitem.author.dept | Plant Pathology and Microbiology | - |
crisitem.author.orcid | 0000-0003-0455-832X | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
顯示於: | 植物病理與微生物學系
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