竹嵌紋病毒之分子生物學─竹嵌紋病毒缺失性RNA之研究
Date Issued
2001
Date
2001
Author(s)
DOI
892311B002092
Abstract
In order to study the defective RNAs (D RNAs) of Bamboo mosaic virus (BaMV), we
chose D20, which had the highest level of accumulation in previous tobacco protoplast assay,
as wild type to construct four frame shift mutants. These mutants with introduced
pre-terminated stop codons had similar RNA sequences and secondary structures, but different
length of open reading frame (ORF). The results indicated that the ORF of D RNAs must be
no less than 99 amino acids in order to maintain their biological activities and the level of
accumulation was correlated with the length of ORF. When the sequences of the ORF was
replaced by BaMV coat protein (CP) gene or foreign EGFP gene, the mutants, D20-CP and
D20-EGFP, were nonviable although their ORF lengths were similar to D20. The above
results indicated that not only the lengths of the ORF but also the sequences within ORF are
important for the accumulation of BaMV D RNA. When D20 were co-inoculated with
D20-CP or D20-EGFP to test if it could complement the replication of both mutants, the
- 2 -
result was negative. According to the experimental results of D20 deletion mutants, the
minimum sequences required for biological activities of BaMV D RNAs are from regions nt
1-551 and nt 936-1118. D20 mutants with nt 388-551 deleted greatly decreased the level of
accumulation and suggested that these deleted sequences maybe contain cis-acting sequence
of replication
Subjects
Bamboo Mosaic
BaMV
Defective RNA
Publisher
臺北市：國立臺灣大學植物病理與微生物學系暨研究所
Type
other
File(s)![Thumbnail Image]()
Loading...
Name
892311B002092.pdf
Size
36.15 KB
Format
Adobe PDF
Checksum
(MD5):471399fa3a657aae0ca912c815908ca5