花生簇葉病菌質體RNA聚合酵素SIGMA FACTOR基因之選殖與分析(II)
Date Issued
2000
Date
2000
Author(s)
林長平
DOI
892313B002034
Abstract
In an effort to elucidate the molecular biology of phytoplasmas, a gene encoding
the primary sigma factor gene of the phytoplasma associated with peanut witches’
broom (PNWB) was cloned and sequnced using PCR-based strategy. A 600-bp
rpoD gene fragment was PCR-amplified and used as a nucleic acid probe for
screening λ ZAP II genomic library of PNWB phytoplasma. The complete
nucleotide sequence of the 1.8 kb insert DNA of pPSF3, one of the in vivo excised
recombinants carrying a complete open reading frame (ORF), was determined. The
ORF encodes a polypeptide contains -10 recognition and binding region, -35
recognition and binding region, core binding region and rpoD box. The gene
organization and the nucleotide sequence in conserved region of the ORF are similar
to other rpoD homologous sigma factor genes. According to the nucleic acid and
amino acid sequence analyses and the results of Northern hybridization analyses, this
gene was identified as a putative sigma factor gene. Based on the results of Southern
hybridization analyses with the 600-bp fragment as a probe, it is suggested that three
rpoD homologous sigma factor genes may exist in PNWB-phytoplasma.
Subjects
phytoplasma
sigma factor gene
Publisher
臺北市：國立臺灣大學植物病理與微生物學系暨研究所
Type
other
File(s)![Thumbnail Image]()
Loading...
Name
892313B002034.pdf
Size
45.88 KB
Format
Adobe PDF
Checksum
(MD5):0c1169a0e2f12687bf2ed9a750314b78