Characterization of Catharanthus roseus Genes Regulated Differentially by Peanut Witches' Broom Phytoplasma Infection
Date Issued
2011
Date
2011
Author(s)
Chen, Wuu-Yang
Abstract
The differential display (DD) strategy was applied to isolate periwinkle (Catharanthus roseus) cDNAs differentially expressed following infection with peanut witches'' broom (PnWB) phytoplasma. Sixty four clones have been selected from differentially expressed cDNA fragments. After screening by reverse northern hybridization, 10 transcripts were selected and sequenced. The expression levels of them was quantified by real-time polymerase chain reaction (PCR), and 7 DD transcripts were identified as ones truly differentially expressed after PnWB phytoplasma infection. One showing homology with phi-1 gene was up-regulated, while the others were down-regulated. Except two of which were predicted protein genes, the other down-regulated genes share homology with psaDa gene, ML domain protein gene, eukaryotic translation initiation factor SUI1 gene, and plastidic aldolase NPALDP1 gene. Homologous genes were further confirmed for 3 DD transcripts by isolating full-length cDNA fragments from the cDNA library constructed in this study. Confirmed genes included ML domain protein gene, translation initiation factor SUI1 gene, and plastidic aldolase NPALDP1 gene, and were mainly involved in innate immune responses, stress responses, and photosynthesis. In the time course experiments, expression levels of these three genes were down-regulated at 6th week after inoculation, and the results agreed with those of differential display. The possible role of these genes in the periwinkle that was infected by PnWB phytoplasma is discussed.
Subjects
differential display
interactions
periwinkle
File(s)![Thumbnail Image]()
Loading...
Name
ntu-100-D91633001-1.pdf
Size
23.54 KB
Format
Adobe PDF
Checksum
(MD5):6ccc825f8719f820a4bbd84fafb8c67f