Studies on Biomonitoring Technology for Ozone
The aims of the study were to explore the applications and mechanisms of the ozone biomonitoring techniques. There were four parts in this study: (1) Black nightshade plantlets from tissue culture are acclimatized in charcoal-filtered air as a bio-indicator for ozone; (2)The use of tobacco T8 indicator plant to monitor the indoor ozone; (3)Factors affecting ozone sensitivity of tobacco Bel-W3 seedlings; and (4) The use of tobacco T8 indicator plant to explore the relationship between ozone injuries and hypersensitive reactions of plants. Results showed: (1) The tissue culture technique is a successful propagation method for providing black nightshades with more sensitive and uniform responses to ozone. An charcoal-filtered or ozone-free ambient air used during plantlet acclimatization can increase the O3 sensitivity. (2) Among the six monitoring sites in the NTU campus, the leaf injury index percentage (LII%) of T8 seedlings were the largest in the copying room of the dormitory. Among the six classrooms in Taipei City, the foliar injuries of the T8 seedlings in Wen-Hua Elementary School were the most serious. (3) Pre-exposure to O3 is a critical factor that decrease the O3 sensitivity and chronically exposed old leaves help protect young leaves from acute injuries when later exposed to O3. (4) Hypersensitive reactions (HR) can be induced by Xanthomons campestris pv. vesicatoria (Xcv ), a non-host bacterium, on two tobacco (Nicotiana tabacum) cultivars with different sensitivity of ozone. While the O3-sensitive tobacco cv. Bel-W3 displayed significantly higher HR intensity than the ozone-resistant tobacco cv. Bel-B. In an age-related study, the older leaves of O3-sensitive tobacco cv. Bel-W3 displayed significantly higher HR intensity than younger leaves. Ozone can be phytotoxic to plants or as an elicitor as well, and both the mechanisms are complex. This study represented that the ozone indicator plants were an ideal model plant to explore the relationship between ozone injuries and hypersensitive reactions of plants.
|Appears in Collections:||植物病理與微生物學系|
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