Synergism in infectivity between Odontoglossum ringspot virus and Cymbidium mosaic virus
Date Issued
2014
Date
2014
Author(s)
Huang, Yu-Wen
Abstract
Synergistic interaction refers to a facilitating effect that the symptom and replication of one or both virus partners increase on mixedly infected host plant. Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV) are two economically important viruses infecting orchids. Mixed infection by these two viruses is a common phenomenon and leads to severe symptoms. Besides, when CymMV and ORSV were inoculated to orchid protoplasts simultaneously, the accumulation of virus increased as compared to the singly infected protoplasts. However, the molecular mechanism of the synergistic interaction is still unknown. In this study, CymMV and ORSV were proved to have synergistic interaction on Nicotiana benthamiana protoplasts which were mixedly inoculated. Additionally, the accumulation of CymMV increased much more than that of ORSV. We proposed that this interaction may be mediated by silencing suppressor of the viruses. Triple gene block 1 protein (TGB1) of CymMV and p126 protein of ORSV which are potential PTGS suppressors were assayed on 16c line GFP transgenic N. benthamiana plants. Our results indicated that p126 possessed stronger suppressor activity than TGB1. When CymMV was inoculated onto N. benthamiana plants transiently expressed with p126, the accumulation of CymMV increased. In contrast, TGB1, which does not possess the ability of silencing suppression, could also enhance the accumulation of ORSV. Accordingly, p126 and TGB1 could mediate the synergistic interaction between CymMV and ORSV but through different mechanisms. Furthermore, we dissected the potential silencing suppression function of each p126 domain. The results indicated that none of these domains could independently suppress PTGS and facilitate the synergistic interaction with CymMV. Hence, we concluded that the ability of silencing suppression was required for ORSV p126 to facilitate CymMV accumulation during mixed infection in N. benthamiana protoplasts.
Subjects
協力作用
齒舌蘭輪斑病毒
蕙蘭嵌紋病毒
基因靜默抑制子
Type
thesis
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