Construction of Transcription Factor Regulatory Network of CrPR1a and CrLOX2 genes in Catharanthus roseus
Date Issued
2014
Date
2014
Author(s)
Lai, Chien-Ming
Abstract
Phytoplasmas are destructive plant pathogens that cause severe loss in agriculture worldwide. However, effective disease management against phytoplasmas have not been developed. Understanding the interactions between plants and phytoplasmas may provide new insights for disease management. CrPR1a can be induced in symptomatic and non-symptomatic shoots after periwinkle leaf yellowing (PLY) phytoplasma infection. CrLOX2, a jasmonate-responsive and biosynthesis gene, is also induced by the phytoplasma infection. Therefore, we aimed to construct transcriptional regulation networks of CrPR1a and CrLOX2 by using virus-induced gene silencing (VIGS). A total of 723 transcription factors in 55 groups were identified from a pre-established transcriptome database. Seven groups of factors, including AP2/EREBP (38), ABI3/VP1 (8), EIL (2), NAC (29), MADS (22), bHLH (38), WRKY (18), that may involve in biotic or abiotic stresses were selected for the screening. Two transcription factors in the AP2/EREBP group were identified to regulate expression of CrPR1a and CrLOX2 respectively. Their expressions under phytoplasma infection, different hormone treatments, and CrNPR1, CrNPR3 gene silencing were examined, and the expression of ERF-34, the potential negative regulator of CrPR1a, was not changed under conditions tested. However, its transcript level was downregulated when ARF-13, a potential positive regulator of CrPR1a, was silenced. ERF-37, a positive regulator of CrLOX2. ERF-37, the potential positive regulator of CrLOX2, was induced after PLY and PnWB phytoplasma infection. We constructed a regulatory network of CrPR1a and CrLOX2 with novel transcription factors. This might help to better understand of plant defense against phytoplasma.
Subjects
CrPR1a
CrLOX2
植物菌質體
病毒誘導基因靜默
轉錄因子
植物抗病
Type
thesis
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