Evaluation of hepatic protection of Agaricus blazei-based herbal extract against CCl4-induced oxidative liver injury
Date Issued
2007
Date
2007
Author(s)
Yen, Chih-Tai
DOI
zh-TW
Abstract
Mushrooms have been used as an important source of nutrition and/or therapy throughout the world since ancient times. Agaricus blazei polysaccharides has been reported to induced with immunomodulatory and antitumor activities. This study was designed to evaluate the effects of Agaricus blazei-based herbal extract on CCl4-induced chronic hepatotoxicity in rats. Male Wistar rats (210 ±10 g) were divided into six groups in this study, including normal control (not treated with CCl4) and groups treated with CCl4, i.e. negative control (only treated with CCl4), positive control (treated with CCl4 and Silymarin), and three experimental groups (treated with CCl4 and low, medium or high dose of Agaricus blazei-based herbal extract, i.e. 0.74, 1.48, or 3.7g/kg bw/day Agaricus blazei-based herbal extract, respectively). On the other hand, rats were administrated with Agaricus blazei-based herbal extract for one week before the administration of CCl4. Rats were treated with Agaricus blazei-based herbal extract once a day until the eighth week of CCl4 treatment. Blood samples were collected for assaying serum biological parameters. The livers were excised for evaluating peroxidation products and antioxidant substances, as well as the activities of antioxidant enzymes. Pathological histology was also performed. The results showed that the Agaricus blazei-based herbal extract significantly (p<0.05) lowered the serum levels of hepatic enzyme markers (alanine aminotransferase, aspartate aminotransferase), inhibited inflammation, necrosis, fatty degeneration and fibrosis. Also reduced the content of the peroxidation product malondialdehyde, and the elevated the content of reduced form of glutathione (GSH). Treatment of rats with Agaricus blazei-based herbal extract could increase the activities of glutathione peroxidase (GPx1), glutathione reductase (GRd) and glutathione S-transferase (GST) in liver when compared with CCl4-treated group (p<0.05). Therefore, the results of this study show that Agaricus blazei-based herbal extract can be proposed to protect the liver against CCl4-induced oxidative damage in rats, and the hepatoprotective effect might be correlated with its antioxidant and free radical scavenger effects.
Subjects
肝臟
巴西洋菇
四氯化碳
麩胱甘肽
抗氧化
解毒代謝
liver
Agaricus blazei
carbon tetrachloride
glutathione
antioxidant property
detoxification
Type
thesis
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