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  4. Inhibitory Effects of Agaricus blazei on U937 Cells and on Metastasis in Mice Injected with CT26 Cells
 
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Inhibitory Effects of Agaricus blazei on U937 Cells and on Metastasis in Mice Injected with CT26 Cells

Date Issued
2004
Date
2004
Author(s)
Chen, Shiuan-Yu
DOI
zh-TW
URI
http://ntur.lib.ntu.edu.tw//handle/246246/56288
Abstract
Abstract Agaricus blazei (AB) is a dietary mushrooms and known for its immunoenhancing, antitumor, antioxidation, antiviral, and anti- mutagenesis actions. In the present study, furiting body of AB was used to investigate the immunoenhancing and antitumor activities in vitro and in vivo. First, 4℃ cold-water extracts, boiled-water extracts, and ethanol (75%) precipitates of both cold- and boiled-water extracts from AB were incubated with human leukemic U937 cells to observe the changes in growth inhibition (%) and differentiation-inducing effect by conditioned medium (CM) method or co-cultivation method, followed by the cell cycle arrest test by a flow cytometry. Results showed that remarkable growth inhibition (about 78 %) was observed when U937 cells were incubated with 400 mg/mL of ethanol precipitates of boiled-water extracts from AB, while cell cycle of U937 cells were arrested at G0/G1 phase when were incubated for 5 days with ethanol precipitates of boiled-water extracts from AB without apoptosis occurrence (< 0.2 %). On the other hand, in vivo, male Balb/c mice, orally administrated with different amounts (200~600 mg/kg/day) of ethanol precipitates of boiled-water extracts from AB, were injected with 2 × 105 CT26 (colon tumor) cells intraveneously via a tail vein to establish pulmonary metastases and then sacrificed after 12 days of inoculation. Metastatic lung nodules in control group were counted to be 121/mouse and experimental group fed with 200, 400, and 600 mg/kg/day were counted to show 36/mouse, 36/mouse, and 55/mouse, respectively, revealing significantly (p < 0.05) the anti-metastatic effect of AB. Histopathologic results in lungs was also consistent with the finding in vivo. Insignificant changes in renal functions and immunohistograph were observed in mice fed with Agaricus blazei at those three dosages.
Subjects
巴西洋菇
轉移
U937
pulmonary metastases
Agaricus blazei
Type
thesis
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