Fractionation and characterization of water-soluble polysaccharides from Agaricus blazei Murill mycelia
Date Issued
2007
Date
2007
Author(s)
Yu, Hui-Ching
DOI
zh-TW
Abstract
Agaricus blazei Murill, is an edible and medicinal mushroom. Literatures have shown that polysaccharides isolated from A. blazei contributed its immunomodulating and antitumor activities. In this thesis, the features of active polysaccharides from A. blazei mycelia fermentation products were studied. Using DEAE-Toyopearl 650M, we separated water-soluble crude polysaccharides from A. blazei Murill mycelia fermentation products into five fractions: neutral polysaccharides (CP-FI), low protein containing polysaccharides (CP-FⅡ), rich protein containing acid polysaccharides (CP-FⅢ), and two polysaccharide-protein complexes (CP-FⅣ and CP-FV). In vitro study, three fractions (CP-FⅢ, CP-FIV, and CP-FV) can significantly stimulate mouse macrophage RAW 264.7 to release TNF-a. The chemical characteristics of CP-FIII were as follows: monosaccharide composition, mannose: glucose: galatose: arabinose: fucose (45：10：30：12：3); uronic acid content, 3.5%; protein content, 3.1%; weight-average molecular weight (Mw), 398.8 kDa; polydispersity, 3.6. According to their molecular weight, FIII was further divided into three fractions: CP-FⅢ-A (442.1 kDa), CP-FⅢ-B (154 kDa) and CP-FⅢ-C (27.4 kDa). Fraction CP-FⅢ-A mainly consisted of galactose, arabinose, mannose and fucose; fraction CP-FⅢ-B mainly consisted of mannose, galactose and arabinose: fraction CP-FⅢ-C mainly consisted of mannose, glucose and galactose. The results indicated that the polysaccharides had bioactivity of TNF-a stimulation on RAW 264.7, a rodent macrophage cell line, were a fucose containing polysaccharide with high molecular weight, and two other protein-glycan complexes.
Subjects
巴西洋菇
多醣
多醣蛋白複合體
免疫調節
菌絲發酵物
Agaricus blazei Murill
polysaccharides
polysaccharides-protein complex
immunomodulating
mycelia fermentation products
Type
thesis
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