Proteomic Analysis of Human Hepatoma G2 Cells Following Treatment with Heterocyclic Amines Trp-P-1
Date Issued
2005
Date
2005
Author(s)
Huang, Da-Wei
DOI
zh-TW
Abstract
Abstract
Heterocyclic amines have been proved to be highly mutagenic and carcinogenic due to the activation of cytochrome P-450 in vivo. Among them, Trp-P-1 is one of the potent toxic heterocyclic amines. In the present study, proteomics on the protein expression of HepG2 cells following treatment with Trp-P-1 were conducted. In vitro, the growth inhibition of HepG2 cells increased in a time-dependent manner after treatment with Trp-P-1 at a level higher than 2 µg/mL. More than 90 % cells were inhibited when treated with 3, 4 or 5 µg/mL Trp-P-1 for 3 days. In proteomic analysis, 6, 9, and 11 protein spots were observed to be increased in quantity by more than 50 % if HepG2 cells were treated with 2 µg/mL Trp-P-1 for 1, 2, and 3 days, respectively. However, protein expression decreased in quantity by more than 50 % were determined to be 3 and 4 protein spots treated with the same amount of Trp-P-1 for 2 and 3 days, respectively. These protein spots were in gel digested by trypsin and analyzed by matrix assisted laser desorption ionization – time of flight ( MALDI-TOF ) mass spectrometry and 4, 10, and 13 proteins were successfully identified in day-1, -2, and -3 sample, respectively. Internet database ( Swiss-Prot ) search indicated that the changes in cell physiology induced by Trp-P-1 were mainly due to the influence of protein functions on energy metabolism cycle and protein structure maintain.
Subjects
雜環胺, 人類肝癌細胞株, 蛋白質體學, 基質輔助雷射吸附游離離子化飛行時間質譜儀
Heterocyclic amines, HepG2, Proteomics, MALDI-Q-TOF
Type
thesis
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